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Transcription profiling of effect of Azinomycin B on cultured Saccharomyces cerevisiae cells

ABSTRACT: Stress responses to DNA damaging agents show characteristic responses. The effect of Azinomycin B upon cultured yeast cells in growth conditions appears to be significant. We used microarrays to detail the global programme of gene expression induced in the stress response to Azinomycin B. Experiment Overall Design: Saccharomyces cerevisiae cells were exposed to selected doses of Azinomycin B for a period of 6 hours incubation for RNA extraction and hybridization on Affymetrix microarrays. We sought to compare stress response states of 100 and 10 ug/ml to control growth.

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Coran MH Watanabe

PROVIDER: E-GEOD-4311 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Cellular effects induced by the antitumor agent azinomycin B.

Kelly Gilbert T GT Liu Chaomin C Smith Roger R Coleman Robert S RS Watanabe Coran M H CM

Chemistry & biology 20060501 5

Studies on the mechanism of action of the antitumor agent azinomycin B in vitro suggest that the drug elicits its lethal effects by the formation of interstrand crosslinks within the major groove of DNA. Here, we demonstrate the biological effects of the drug in vivo. Fluorescence imaging revealed localization of azinomycin B in the nuclear region of yeast. Moreover, experiments with oligonucleotide microarrays examined the effects of the drug across the yeast transcriptome. The results demonstr ...[more]

PMID: 16720269

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