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A novel phosphate-starvation response in fission yeast requires the endocytic function of Myosin I (RNA-seq)


ABSTRACT: S. pombe Myosin I (MyoI) plays a key role in cytoskeletal functions, in particular it is part of the endocytotic machinery. Here we show a novel role for MyoI in regulating the response to low phosphate stress. Deletion of MyoI led to an increased proliferation in low phosphate media when compared to a control strain; mutations in other proteins involved in the early steps of endocytosis, such as ARP2 and Sla2/END4, led to the same phenotype. We show that once cells are deprived of phosphate they undergo clear morphological changes, slow down growth but retain viability. This is reminiscent of a quiescence response, and it is completely abolished when MyoI is absent. Transcriptomic analysis revealed a wide perturbation of gene expression upon phosphate starvation, with rapid induction of stress-regulated genes. However, Æmyo1 cells displayed little or negligible effects at gene expression level when they were phosphate-starved. Using a combination of super resolution microscopy and genetic approaches we pinpointed the specific function of MyoI in endocytosis as critical for the response to low phosphate stress. Furthermore, introduction of point-mutated MyoI (with ablated endocytic function) into a ÆmyoI background failed to restore phosphate stress response. Taken together, our data indicate how endocytosis, and in particular MyoI, play a pivotal role in mediating cellular response to nutrients, bridging the external environment and internal molecular functions of the cells. RNA-seq transcriptomic analysis in control and ∆myo1 S.pombe strains comparing gene expression profiles at 4 and 10 hours after phosphate depletion.

ORGANISM(S): Schizosaccharomyces pombe

SUBMITTER: Felix Krueger 

PROVIDER: E-GEOD-65579 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

A new phosphate-starvation response in fission yeast requires the endocytic function of myosin I.

Petrini Edoardo E   Baillet Victoire V   Cridge Jake J   Hogan Cassandra J CJ   Guillaume Cindy C   Ke Huiling H   Brandetti Elisa E   Walker Simon S   Koohy Hashem H   Spivakov Mikhail M   Varga-Weisz Patrick P  

Journal of cell science 20150907 20


Endocytosis is essential for uptake of many substances into the cell, but how it links to nutritional signalling is poorly understood. Here, we show a new role for endocytosis in regulating the response to low phosphate in Schizosaccharomyces pombe. Loss of function of myosin I (Myo1), Sla2/End4 or Arp2, proteins involved in the early steps of endocytosis, led to increased proliferation in low-phosphate medium compared to controls. We show that once cells are deprived of phosphate they undergo a  ...[more]

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