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Expression data from WT and E2A-deficient murine DN2 cells

ABSTRACT: The E2A transcription factors promote the development of thymus-seeding cells but it remains unknown whether these proteins play a role in T lymphocyte lineage specification or commitment. By examining E2A-dependent genes in developing T cells, we will address whether these proteins are involved in these processes. DN2 cells from WT and E2A-deficient murine fetal thymi were FACS sorted. Subsequently RNA was extracted, labelled and hybridized to Affymetrix microarrays. The goal of this study is to investigate E2A-dependent genes in developing T cells

ORGANISM(S): Mus musculus

SUBMITTER: Barbara Kee

PROVIDER: E-GEOD-43224 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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E2A transcription factors limit expression of Gata3 to facilitate T lymphocyte lineage commitment.

Xu Wei W Carr Tiffany T Ramirez Kevin K McGregor Stephanie S Sigvardsson Mikael M Kee Barbara L BL

Blood 20130107 9

The E2A transcription factors promote the development of thymus-seeding cells, but it remains unknown whether these proteins play a role in T lymphocyte lineage specification or commitment. Here, we showed that E2A proteins were required to promote T-lymphocyte commitment from DN2 thymocytes and to extinguish their potential for alternative fates. E2A proteins functioned in DN2 cells to limit expression of Gata3, which encodes an essential T-lymphocyte transcription factor whose ectopic expressi ...[more]

PMID: 23297135

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