Unknown,Transcriptomics,Genomics,Proteomics

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Transcriptomic profile of Mycobacteriophage Giles


ABSTRACT: The gene expression during early (30min) and late (2.5hrs) infection of Mycobacteriophage Giles was analyzed, as well as in a Giles lysogen. Total RNA was isolated, depleted of rRNA and prepped for sequencing (Illumina) during early and late infection of Giles and a lysogen.

ORGANISM(S): Mycobacterium phage Giles

SUBMITTER: Rebekah Dedrick 

PROVIDER: E-GEOD-43434 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Functional requirements for bacteriophage growth: gene essentiality and expression in mycobacteriophage Giles.

Dedrick Rebekah M RM   Marinelli Laura J LJ   Newton Gerald L GL   Pogliano Kit K   Pogliano Joseph J   Hatfull Graham F GF  

Molecular microbiology 20130408 3


Bacteriophages represent a majority of all life forms, and the vast, dynamic population with early origins is reflected in their enormous genetic diversity. A large number of bacteriophage genomes have been sequenced. They are replete with novel genes without known relatives. We know little about their functions, which genes are required for lytic growth, and how they are expressed. Furthermore, the diversity is such that even genes with required functions - such as virion proteins and repressor  ...[more]

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