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Genome-wide profiling of Zfp335 binding sites in thymocytes

ABSTRACT: The generation of naM-CM-/ve T lymphocytes is critical for immune function yet the mechanisms governing their maturation remain incompletely understood. We have identified a mouse mutant, bloto, that harbors a hypomorphic mutation in the zinc finger protein Zfp335. Mutant blt/blt mice exhibit a naM-CM-/ve T cell deficiency due to an intrinsic developmental defect that begins to manifest in the thymus and continues into the periphery, affecting T cells that have recently undergone thymic egress. Zfp335 binds to promoter regions via a consensus motif, and its target genes are enriched in categories related to protein metabolism, mitochondrial function and transcriptional regulation. Restoring the expression of one target, Ankle2, partially rescues T cell maturation. Our findings identify Zfp335 as a transcription factor and essential regulator of late-stage intrathymic and post-thymic T cell maturation. 4 Zfp335 ChIP-seq samples and 2 input samples with WT vs. blt/blt thymocytes.

ORGANISM(S): Mus musculus

SUBMITTER: Brenda Han 

PROVIDER: E-GEOD-58333 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Zinc finger protein Zfp335 is required for the formation of the naïve T cell compartment.

Han Brenda Y BY   Wu Shuang S   Foo Chuan-Sheng CS   Horton Robert M RM   Jenne Craig N CN   Watson Susan R SR   Whittle Belinda B   Goodnow Chris C CC   Cyster Jason G JG  

eLife 20141024

The generation of naïve T lymphocytes is critical for immune function yet the mechanisms governing their maturation remain incompletely understood. We have identified a mouse mutant, bloto, that harbors a hypomorphic mutation in the zinc finger protein Zfp335. Zfp335(bloto/bloto) mice exhibit a naïve T cell deficiency due to an intrinsic developmental defect that begins to manifest in the thymus and continues into the periphery, affecting T cells that have recently undergone thymic egress. The e  ...[more]

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