Expression analysis of Mycobacterium avium subsp. paratuberculosis K-10 within macrophage cell line

ABSTRACT: Once entering the cell, M. avium subsp.paratuberculosis is known to survive harsh microenvironments, especially those inside activated macrophages. To improve our understanding of M. avium subsp.paratuberculosis pathogenesis, we examined the phagosome maturation associated with transcriptional responses of M. avium subsp.paratuberculosis during macrophage infection. Monitoring cellular markers, only live M. avium subsp.paratuberculosis bacilli were able to prevent phagosome maturation and reduce its acidification. On the transcriptional level, over 300 of M. avium subsp.paratuberculosis genes were significantly, differentially regulated in both naM-CM-/ve and IFN-M-NM-3-activated macrophages. These genes include the sigma factor H (sigH) that was shown to be important during persistent

ORGANISM(S): Mycobacterium avium subsp. paratuberculosis K-10

SUBMITTER: CHIA-WEI WU 

PROVIDER: E-GEOD-43645 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress