Dataset Information

Identification of quantitative proteomic differences between mycobacterial lineages with altered virulence

ABSTRACT: Mycobacterium tuberculosis is the causitive agent of TB, which is a global epidemic that has claimed millions of lives. Some clinical strains of M.tb have significantly more severe clinical outcomes, while some other mycobacteria ordinarily do not cause disease in humans. The biological processes underpinning this difference are poorly understood. Thus, this project aimed to identify proteomic differences between more virulent strains of the MTBC (Beijing, J37Rv, CAS and LAM) vs those strains that only cause disease in severely immune compromised humans (Bovis, Smegmatis and Avium). We hypothesise that these differences may offer insight into the molecular mechanisms by which M.tb has become more virulent by evading drug treatment or evading the host immune system more effectively.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Mycobacterium Tuberculosis Cas Mycobacterium Bovis Bcg Mycobacterium Tuberculosis Lam7 Mycobacterium Avium 100 Mycobacterium Bovis Str. Ravenel Mycobacterium Tuberculosis Str. Beijing/w Bt1 Mycobacterium Tuberculosis H37rv

TISSUE(S): Cell Culture

SUBMITTER: Bridget Calder

LAB HEAD: Jonathan Blackburn

PROVIDER: PXD004165 | Pride | 2016-05-23

REPOSITORIES: pride

ACCESS DATA

Dataset's files

Source:

Items per page:

1 - 5 of 68

Publications

Identification of Quantitative Proteomic Differences between Mycobacterium tuberculosis Lineages with Altered Virulence.

Peters Julian S JS Calder Bridget B Gonnelli Giulia G Degroeve Sven S Rajaonarifara Elinambinina E Mulder Nicola N Soares Nelson C NC Martens Lennart L Blackburn Jonathan M JM

Frontiers in microbiology 20160531

Evidence currently suggests that as a species Mycobacterium tuberculosis exhibits very little genomic sequence diversity. Despite limited genetic variability, members of the M. tuberculosis complex (MTBC) have been shown to exhibit vast discrepancies in phenotypic presentation in terms of virulence, elicited immune response and transmissibility. Here, we used qualitative and quantitative mass spectrometry tools to investigate the proteomes of seven clinically-relevant mycobacterial strains-four ...[more]

PMID: 27303394

Similar Datasets

Project description:Phosphorylation is a regulatory mechanism by which intracellular apicomplexan parasites control the process of invading and modifying host cells. However, the sporulated oocysts’ phosphoprotein repertoires between virulent and avirulent strains of Toxoplasma gondii and the underlying mechanisms contributing to virulence is still a mystery. A quantitative analysis of the sporulated oocysts’ phosphoproteome profile of T. gondii virulent and avirulent strains belonging to different genotypes was conducted to reveal phosphoprotein expression patterns that contribute to the virulence of a particular phenotype. Phosphopeptides from the sporulated oocysts of the virulent strain PYS (Chinese ToxoDB#9) and the avirulent strain type II (PRU strain) were enriched by titanium dioxide (TiO2) affinity chromatography and quantified using iBT technology. A total of 10,645 unique phosphopeptides, 8,181 nonredundant phosphorylation sites and 2,792 phosphoproteins were identified. The quantitative analysis identified 4,129 differentially expressed phosphopeptides (DEPs) between sporulated oocysts of the PYS strain and PRU strain (|log1.5 fold change| > 1 and P < 0.05), including 2,485 upregulated and 1,644 downregulated phosphopeptides. Motif analysis identified 24 motifs from the upregulated phosphorylated peptides including 22 serine motifs and two threonine motifs (TPE and TP), and 15 motifs from the downregulated phosphorylated peptides including 12 serine motifs and three threonine motifs (TP, RxxT and KxxT) in PYS strain when comparing PYS/PRU. Several kinases were consistent with motifs of overrepresented phosphopeptides, such as PKA, PKG, CKII, IKK, MAPK, EGFR, INSR, Jak, Syk, Src, Ab1. GO analysis, KEGG pathway analysis and STRING analysis revealed differentially expressed phosphoproteins (DEPs) exhibiting significant functional properties. Kinase associated network analysis showed that AGC kinase had the greatest connected peptides. Our findings reveal significant difference in phosphopeptides of sporulated oocysts between virulent and avirulent T. gondii strains, which provides solid foundation for further exploring the mechanisms contributing to the virulence of T. gondii.

			Action	DRS
	Avium_TP_Top20CID_120min_01.raw	Raw
	Avium_TP_Top20CID_120min_02.raw	Raw
	Aviumall.mzXML	Mzxml
	BCGCF_1.raw	Raw
	BCGCF_2.raw	Raw