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MiRNA 135b regulation by Il1r1 during inflammation

ABSTRACT: RT-PCR analysis of inflammatory cytokines and receptors following Il1alpha treatment of pEGP-miR-135b over-expressing cells compard to controls (pEGP-miR-null) using pathway-specific PCR arrays for the evaluation of mouse inflammatory cytokines and receptors (#PAMM-011D). qPCR array gene expression profiling. This experiment examined the transcriptional response of NIH3T3 cells over-expressing pEGP-miR-135b following IL1alpha treatment. Total RNA (n = 3 per group) was used with SABiosciences pathway-specific PCR arrays for inflammatory cytokines and receptors (#PAMM-011D).

ORGANISM(S): Mus musculus

SUBMITTER: Sarah Labib

PROVIDER: E-GEOD-43683 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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IL-1 receptor regulates microRNA-135b expression in a negative feedback mechanism during cigarette smoke-induced inflammation.

Halappanavar Sabina S Nikota Jake J Wu Dongmei D Williams Andrew A Yauk Carole L CL Yauk Carole L CL Stampfli Martin M

Journal of immunology (Baltimore, Md. : 1950) 20130225 7

Although microRNA-135b (miR-135b) is known to be associated with cancer, with recent work showing that it is massively induced in the pulmonary tissues of mice challenged with nanoparticles suggests a critical role for this microRNA in mediating inflammatory response. In this study, we investigated the expression and function of miR-135b in mice exposed to cigarette smoke or nontypeable Haemophilus influenzae (NTHi). Exposure to both cigarette smoke and NTHi elicited robust lung inflammation, bu ...[more]

PMID: 23440414

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miRNA 135b regulation of inflammation

Project description:RT-PCR analysis of inflammatory cytokines and receptors in pEGP-miR-135b over-expressing cells compard to controls (pEGP-miR-null) using pathway-specific PCR arrays for the evaluation of mouse inflammatory cytokines and receptors (#PAMM-011D). qPCR array gene expression profiling. This experiment examined the transcriptional response of NIH3T3 cells over-expressing pEGP-miR-135b. Total RNA (n = 3 per group) was used with SABiosciences pathway-specific PCR arrays for inflammatory cytokines and receptors (#PAMM-011D).

2013-01-31 | E-GEOD-43681 | biostudies-arrayexpress

miRNA 135b regulation of inflammation

2013-01-31 | GSE43681 | GEO

miRNA 135b regulation by Il1r1 during inflammation

Project description:RT-PCR analysis of inflammatory cytokines and receptors following Il1alpha treatment of pEGP-miR-135b over-expressing cells compard to controls (pEGP-miR-null) using pathway-specific PCR arrays for the evaluation of mouse inflammatory cytokines and receptors (#PAMM-011D).

2013-01-31 | GSE43683 | GEO

Interleukin 1 receptor regulates miRNA-135b expression in a negative feedback mechanism during cigarette smoke-induced inflammation

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2013-01-31 | E-GEOD-43688 | biostudies-arrayexpress

Real-time quantitative PCR analysis of mouse lung epithelial cells 12 (MLE-12)

Project description:MLE12 cells were treated with 8-oxoguanine DNA glycosylase 1 (OGG1) inhibitor with or without TNF to evaluate its effect on pro-inflammatory gene expression. We used SA Biosciences Mouse Inflammatory Cytokines & Receptors PCR Array (PAMM-011Z) to quantitate inflammatory gene expression dependent on OGG1 .

2018-07-06 | GSE106785 | GEO

miR-135b exerts metastatic activity in the ErbB2 driven mammary carcinogenesis

Project description:In view of the roles played by miRNAs in cancer progression, we performed a microarray analysis aimed at identifying miR-135b target genes in breast cancer cells. Detection of transcripts affected by silencing of mir-135b was done using a time course at 24, 48 and 72 h both transfecting cell with mir-135b specific miRIDIAN microRNA Hairpin Inhibitor or miRIDIAN microRNA Hairpin Inhibitor negative control. Each experimental condition was repeated three times and analysed on on MOUSEREF-8 V2 Illumina bead chips. Genome studio was used to generate raw intensity signal without normalization. Data log2 transformation and loess normalization was performed with oneChannelGUI. Data were filtered using an IQR filter (i.e. only genes characterized by an IQR > 0.25, were retained for further analysis). Detection of differentially expressed transcripts was also performed with oneChannelGUI, using masigpro package with default parameters. Data were visualized by hierarchical clustering, as log2 ratio between anti-miR135b and the negative control using TMEV software. The subset of transcripts characterized by an increase in expression as consequence of a reduction of expression of miR135b were selected. MicroRNA target data for mir135b were retrieved from miranda database (microRNAsmiranda.mouse_predictions_S_C_aug2010.txt, http://www.microrna.org/microrna/home.do). Miranda data and transcripts increasing their expression upon silencing of miR135b were intersected to find the genes in common. Detection of transcripts affected by silencing of mir-135b was done using a time course at 24, 48 and 72 h both transfecting cell with mir-135b specific miRIDIAN microRNA Hairpin Inhibitor or miRIDIAN microRNA Hairpin Inhibitor negative control.

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Real-time quantitative PCR analysis of the expression of neurotransmitter receptor and regulator genes in 4 week-old male MeCP2 A140V mutant mouse cerebellum

Project description:The goal of this study was to identify cerebellar gene expression differences between mutant MeCP2 A140V mice and their wild type littermates.The PCR array used for these experiments was the Mouse Neurotransmitter Receptors and Regulators Array (Catalog# PAMM-060A) from SABiosciences. Mice used in this study were 4 week old males. Total RNA was extracted from whole cerebella of 4 week old male MeCP2 A140V hemizygous mutant mice and their wild type littermates. For this study, cerebella from 3 mice of each genotype were used and RNA from the cerebellum of each mouse was analyzed separately (ie., triplicate biological replicates). The RNA was purified to remove genomic DNA and was then reverse transcribed for use in quantitative RT-PCR analysis of gene expression using the SABiosciences RT2 profiler PCR array system. The PCR array used for these experiments was the Mouse Neurotransmitter Receptors and Regulators Array (Catalog# PAMM-060A).

2015-02-01 | E-GEOD-35693 | biostudies-arrayexpress

Real-time quantitative PCR analysis of the expression of neurotransmitter receptor and regulator genes in 2 week-old male MeCP2 A140V mutant mouse cerebellum

Project description:The goal of this study was to identify cerebellar gene expression differences between mutant MeCP2 A140V mice and their wild type littermates.The PCR array used for these experiments was the Mouse Neurotransmitter Receptors and Regulators Array (Catalog# PAMM-060A) from SABiosciences. Mice used in this study were 2 week old males. Total RNA was extracted from whole cerebella of 2 week old male MeCP2 A140V hemizygous mutant mice and their wild type littermates. For this study, cerebella from 3 mice of each genotype were used and RNA from the cerebellum of each mouse was analyzed separately (ie., triplicate biological replicates). The RNA was purified to remove genomic DNA and was then reverse transcribed for use in quantitative RT-PCR analysis of gene expression using the SABiosciences RT2 profiler PCR array system. The PCR array used for these experiments was the Mouse Neurotransmitter Receptors and Regulators Array (Catalog# PAMM-060A).

2015-02-01 | E-GEOD-35692 | biostudies-arrayexpress

Label free proteomic analysis of CAL62/miR-331-5p cells

Project description:The ectopic expression of miR-331-5p suppresses TC cell (CAL62 and TPC-1) malignant behavior was demonstrate

2024-05-24 | PXD039884 | Pride

Real-time quantitative PCR analysis of the expression of neurotransmitter receptor and regulator genes in 4 week old male MeCP2 A140V mutant mouse brain cortex

Project description:The goal of this study was to identify brain cortex gene expression differences between mutant MeCP2 A140V mice and their wild type littermates.The PCR array used for these experiments was the Mouse Neurotransmitter Receptors and Regulators Array (Catalog# PAMM-060A) from SABiosciences. Mice used in this study were 4 week old males. Total RNA was extracted from the somatosensory/motor cortex of 4 week old male MeCP2 A140V hemizygous mutant mice and their wild type littermates. For this study, brain tissue from 3 mice of each genotype was used and RNA from the cortex of each mouse was analyzed separately (ie., triplicate biological replicates). The RNA was purified to remove genomic DNA and was then reverse transcribed for use in quantitative RT-PCR analysis of gene expression using the SABiosciences RT2 profiler PCR array system. The PCR array used for these experiments was the Mouse Neurotransmitter Receptors and Regulators Array (Catalog# PAMM-060A).

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