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MiRNA 135b regulation of inflammation

ABSTRACT: RT-PCR analysis of inflammatory cytokines and receptors in pEGP-miR-135b over-expressing cells compard to controls (pEGP-miR-null) using pathway-specific PCR arrays for the evaluation of mouse inflammatory cytokines and receptors (#PAMM-011D). qPCR array gene expression profiling. This experiment examined the transcriptional response of NIH3T3 cells over-expressing pEGP-miR-135b. Total RNA (n = 3 per group) was used with SABiosciences pathway-specific PCR arrays for inflammatory cytokines and receptors (#PAMM-011D).

ORGANISM(S): Mus musculus

SUBMITTER: Sarah Labib

PROVIDER: E-GEOD-43681 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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IL-1 receptor regulates microRNA-135b expression in a negative feedback mechanism during cigarette smoke-induced inflammation.

Halappanavar Sabina S Nikota Jake J Wu Dongmei D Williams Andrew A Yauk Carole L CL Yauk Carole L CL Stampfli Martin M

Journal of immunology (Baltimore, Md. : 1950) 20130225 7

Although microRNA-135b (miR-135b) is known to be associated with cancer, with recent work showing that it is massively induced in the pulmonary tissues of mice challenged with nanoparticles suggests a critical role for this microRNA in mediating inflammatory response. In this study, we investigated the expression and function of miR-135b in mice exposed to cigarette smoke or nontypeable Haemophilus influenzae (NTHi). Exposure to both cigarette smoke and NTHi elicited robust lung inflammation, bu ...[more]

PMID: 23440414

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