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Tissue macrophage subsets derived from regenerating muscle

ABSTRACT: Muscle injury was elicited by cardiotoxin injection into the tibialis anterior muscle. Macrophages were isolated 2 days post-injury from the regenerating muscle. We used microarray to obtain global gene expression data of muscle-derived tissue macrophage subsets. Tissue macrophages were collected from regenerating muscle samples of three animals, Ly6C+ F4/80low and Ly6C- F4/80high macrophage subsets were sorted. The global gene expression patterns of distinct macrophage subsets were analyzed on Affymetrix microarrays.

ORGANISM(S): Mus musculus

SUBMITTER: Szilard Poliska

PROVIDER: E-GEOD-44057 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Tissue LyC6- macrophages are generated in the absence of circulating LyC6- monocytes and Nur77 in a model of muscle regeneration.

Varga Tamas T Mounier Rémi R Gogolak Peter P Poliska Szilard S Chazaud Bénédicte B Nagy Laszlo L

Journal of immunology (Baltimore, Md. : 1950) 20131016 11

There are several open questions regarding the origin, development, and differentiation of subpopulations of monocytes, macrophages (MFs), and dendritic cells. It is a particularly intriguing question how circulating monocyte subsets develop and contribute to the generation of steady-state and inflammatory tissue MF pools and which transcriptional mechanisms contribute to these processes. In this study, we took advantage of a genetic model in which LyC6(-) circulating monocyte development is sev ...[more]

PMID: 24133167

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Mus musculus

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| PRJNA188463 | ENA

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