Unknown,Transcriptomics,Genomics,Proteomics

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Profiling of microRNA expression in hepatocellular carcinoma cells with overexpression of G protein alpha 12 active mutant

ABSTRACT: Heterotrimeric guanine nucleotide-binding proteins (G proteins) transmit extracellular signals from cell surface G protein-coupled receptors to intracellular effector molecules. Attention has been paid to the GM-NM-112 family members because they mediate cell invasion, migration, and tumorigenesis. In our findings, GM-NM-112 levels were higher in human HCCs than non-tumorous liver tissues. Array analyses using Huh7 cells stably transfected with GM-NM-112QL (an active mutant form) enabled us to extract the microRNAs dysregulated by GM-NM-112 active mutant. Of them, miR-122 was most greatly decreased. In addition, we found decreases of miR-200b/a, miR-192/215 levels. Dysregulation of the microRNAs changed the levels of key proteins associated with HCC cell migration/invasion. Huh7 hepatoma cells were transfected with pCMV or the plasmid encoding for activated mutant of GM-NM-112 (GM-NM-112QL). Stable transfectants were selected by incubating the cells in culture medium containing Geneticin for 3 weeks. We performed a miRNA microarray for the identification of global microRNA expression changes using WT-Huh7 and Huh7-GM-NM-112QL cells. RNA for each set was harvested from quadruple plates.

ORGANISM(S): Homo sapiens

SUBMITTER: Sang Geon Kim

PROVIDER: E-GEOD-44079 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Profiling of microRNA expression in hepatocellular carcinoma cells with overexpression of G protein alpha 12 active mutant

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