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Key Role of MicroRNA in the Regulation of Granulocyte Macrophage Colony-stimulating Factor Expression in Murine Alveolar Epithelial Cells (AEC) during Oxidative Stress

ABSTRACT: A genome wide microarray identified 19 candidate miRNA altered in primary AEC during oxidative stress with reversal by treatment with GM-CSF. Three of these microRNA (miR 133a, miR133a* and miR133b) are also predicited to bind the GM-CSF 3 UTR. 4 samples. Primary murine alveolar epithelial cells were isolated and subjected to room air or 80% oxygen in the presence or absence of recombinant murine GM-CSF (20 ng/ml). Total RNA was extracted and quality checked (260/280>2 and 260/230 > 1.6). Mouse genome microRNA analysis (MAM3200) was performed by SABioscience.

ORGANISM(S): Mus musculus

SUBMITTER: Jesse Rowley

PROVIDER: E-GEOD-56687 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Key role of microRNA in the regulation of granulocyte macrophage colony-stimulating factor expression in murine alveolar epithelial cells during oxidative stress.

Sturrock Anne A Mir-Kasimov Mustafa M Baker Jessica J Rowley Jesse J Paine Robert R

The Journal of biological chemistry 20131226 7

GM-CSF is an endogenous pulmonary cytokine produced by normal alveolar epithelial cells (AEC) that is a key defender of the alveolar space. AEC GM-CSF expression is suppressed by oxidative stress through alternations in mRNA turnover, an effect that is reversed by treatment with recombinant GM-CSF. We hypothesized that specific microRNA (miRNA) would play a key role in AEC GM-CSF regulation. A genome-wide miRNA microarray identified 19 candidate miRNA altered in primary AEC during oxidative stre ...[more]

PMID: 24371146

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2012-04-01 | E-GEOD-34827 | biostudies-arrayexpress

Loss of GM-CSF-dependent instruction of alveolar macrophages in COVID-19 provides a rationale for inhaled GM-CSF treatment (RNA-Seq)

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SIGNAL INTEGRATION AND TRANSCRIPTIONAL REGULATION OF THE INFLAMMATORY RESPONSE MEDIATED BY THE GM-/M-CSF SIGNALING AXIS IN HUMAN MONOCYTES [RNA-seq]

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