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Time dependent expression profiles of mRNAs in rat milk

ABSTRACT: We have reported that mRNAs are present in bovine milk. But, it is unknown the roles of milk mRNAs. To clarify the roles of milk mRNAs, experimental animal such as rat study would be needed. However, it is unclear whether rat milk also contains mRNAs. Thus, we have employed microarray to show that rat milk also contains mRNAs. Milk samples were obtained at day 2, 9, and 16 postpartum from 3 rats. And serum samples were obtained from dam rats (n=3) at day 21 postpartum.

ORGANISM(S): Rattus norvegicus

SUBMITTER: Hirohisa Izumi

PROVIDER: E-GEOD-44112 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Time-dependent expression profiles of microRNAs and mRNAs in rat milk whey.

Izumi Hirohisa H Kosaka Nobuyoshi N Shimizu Takashi T Sekine Kazunori K Ochiya Takahiro T Takase Mitsunori M

PloS one 20140212 2

Functional RNAs, such as microRNA (miRNA) and mRNA, are present in milk, but their roles are unknown. To clarify the roles of milk RNAs, further studies using experimental animals such as rats are needed. However, it is unclear whether rat milk also contains functional RNAs and what their time dependent expression profiles are. Thus, we prepared total RNA from whey isolated from rat milk collected on days 2, 9, and 16 postpartum and analyzed using microarrays and quantitative PCR. The concentrat ...[more]

PMID: 24533154

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