Unknown,Transcriptomics,Genomics,Proteomics

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Time dependent expression profiles of microRNAs in rat milk


ABSTRACT: We have reported that microRNAs are present in human and bovine milk. But, it is unknown the roles of milk miRNAs. To clarify the roles of milk miRNAs, experimental animal such as rat study would be needed. However, it is unclear whether rat milk also contains miRNAs. Thus, we have employed microarray to show that rat milk also contains miRNAs. Milk samples were obtained at day 2, 9, and 16 postpartum from 3 rats. And serum samples were obtained from dam rats (n=3) at day 21 postpartum.

ORGANISM(S): Rattus norvegicus

SUBMITTER: Hirohisa Izumi 

PROVIDER: E-GEOD-44113 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Time-dependent expression profiles of microRNAs and mRNAs in rat milk whey.

Izumi Hirohisa H   Kosaka Nobuyoshi N   Shimizu Takashi T   Sekine Kazunori K   Ochiya Takahiro T   Takase Mitsunori M  

PloS one 20140212 2


Functional RNAs, such as microRNA (miRNA) and mRNA, are present in milk, but their roles are unknown. To clarify the roles of milk RNAs, further studies using experimental animals such as rats are needed. However, it is unclear whether rat milk also contains functional RNAs and what their time dependent expression profiles are. Thus, we prepared total RNA from whey isolated from rat milk collected on days 2, 9, and 16 postpartum and analyzed using microarrays and quantitative PCR. The concentrat  ...[more]

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