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KDM4A regulated genes in human squamous carcinoma cells

ABSTRACT: To establish a robust cellular model system for screening genes associated with cell invasion, we over-expressed the oncogenic translocated promoter region (Tpr)-MET proteins in SCC23 cells (SCC23/MET). Using a functional siRNA screen, we identified that the histone demethylase KDM4A played a critical role in the invasive growth and metastasis of SCC mediated by the Oncogenic MET. To investigate the molecular mechanism through which KDM4A inhibit the tumor cell invasion, we knock-down KDM4A in SCC23/MET cell and performed a gene microarray to examine which genes may be regulaged by kDM4A. We generated two stable cell lines. one was infected with virus containing scramble and another infected with virus infected with virus containing KDM4A shRNA. Total RNA were extracted from these two cell lines and subject to microarray.

ORGANISM(S): Homo sapiens

SUBMITTER: xiangming ding 

PROVIDER: E-GEOD-44238 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Epigenetic activation of AP1 promotes squamous cell carcinoma metastasis.

Ding Xiangming X   Pan Hongya H   Li Jiong J   Zhong Qi Q   Chen Xiaohong X   Dry Sarah M SM   Wang Cun-Yu CY  

Science signaling 20130430 273

The transcription factor AP1 (activating protein 1), a heterodimer of the JUN and FOS proteins, promotes the invasive growth and metastasis of various tumors such as squamous cell carcinoma (SCC), breast cancer, and melanoma. AP1 activity is transcriptionally induced through a positive feedback loop. We identified the histone demethylase KDM4A (lysine-specific demethylase 4A) as a key epigenetic priming factor in this positive feedback loop. KDM4A contributed to the induction of genes encoding t  ...[more]

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