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Transcription profiling of rat duodenum during immobilization

ABSTRACT: In the present study, we examined duodenal gene expression in rats subjected to immobilization, to elucidate the mechanism of the stress response in the duodenum. Experiment Overall Design: Total RNA was extracted from the duodenal mucosa with an acid guanidinium thiocyanate-phenol-chloroform mixture. Total RNA was analyzed using the rat genome U34A microarray (Affymetrix). Gene intensity information was converted to a mean intensity for each gene using the manufacturerâs proprietary software (version 4.0; Affymetrix), which includes routines for data filtering and normalization.

ORGANISM(S): Rattus norvegicus

SUBMITTER: Tadatoshi Sato

PROVIDER: E-GEOD-4426 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Restraint stress alters the duodenal expression of genes important for lipid metabolism in rat.

Sato Tadatoshi T Yamamoto Hironori H Sawada Naoki N Nashiki Kunitaka K Tsuji Mitsuyoshi M Muto Kazusa K Kume Hisae H Sasaki Hajime H Arai Hidekazu H Nikawa Takeshi T Taketani Yutaka Y Takeda Eiji E

Toxicology 20060814 3

Stress, such as trauma and injury, is known to cause transcriptional changes in various tissues; however, there is little information on tissue-specific gene expression in response to stress. Here, we have examined duodenal gene expression in rats subjected to whole-body immobilization in order to elucidate the mechanism underlying the stress response in the duodenum--one of the tissues that is most sensitive to external stress. DNA microarray analysis revealed that the immobilization for 2 week ...[more]

PMID: 16962226

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