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Genome-wide maps of DNA cytosine methylation state in during normal differentiation commitment in hematopoietic stem cells (HSC) and in Special AT-rich sequence-binding protein1 (Satb1) - deficient HSC

ABSTRACT: Our data show Satb1 deficiency leads to alterations in DNA cytosine methylation and a commitment-primed epigenetic state in HSCs. Examination of DNA cytosine methylation in wild type HSC and differentiation-committed progenitors as well as in wild type HSC and HSC lacking Satb1 (n=2 each).

ORGANISM(S): Mus musculus

SUBMITTER: Boris Bartholdy

PROVIDER: E-GEOD-44304 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Satb1 regulates the self-renewal of hematopoietic stem cells by promoting quiescence and repressing differentiation commitment.

Will Britta B Vogler Thomas O TO Bartholdy Boris B Garrett-Bakelman Francine F Mayer Jillian J Barreyro Laura L Pandolfi Ashley A Todorova Tihomira I TI Okoye-Okafor Ujunwa C UC Stanley Robert F RF Bhagat Tushar D TD Verma Amit A Figueroa Maria E ME Melnick Ari A Roth Michael M Steidl Ulrich U

Nature immunology 20130407 5

How hematopoietic stem cells (HSCs) coordinate the regulation of opposing cellular mechanisms such as self-renewal and differentiation commitment remains unclear. Here we identified the transcription factor and chromatin remodeler Satb1 as a critical regulator of HSC fate. HSCs lacking Satb1 had defective self-renewal, were less quiescent and showed accelerated lineage commitment, which resulted in progressive depletion of functional HSCs. The enhanced commitment was caused by less symmetric sel ...[more]

PMID: 23563689

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