Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Androgen receptor coregulators baseline comparisons

ABSTRACT: LNCaP cells are an established androgen receptor expressing prostate carcinoma cell line. Human foreskin fibroblasts also expressing the androgen receptor were obtained from phenotypic normal male individuals. Cells were cultured either at confluency leading to G0 cell cycle state or while they were proliferating. Cells were either untreated, or treated with dihydrotestosterone (DHT) or ethanol (ETOH) which also served as the solvent for the DHT. All experimental RNA samples derived from the untreated or treated cell lines were hybridized on cDNA arrays against a common reference. This reference was composed out of common reference CRG (50%) and out of fibroblast RNA (50%). This reference is also called "mixed reference" in the description of the 26 individual experiments. A strain or line experiment design type assays differences between multiple strains, cultivars, serovars, isolates, lines from organisms of a single species. Compound Based Treatment: dihydrotestosterone (DHT) or ethanol (ETOH = solvent) Cell Line: genital fibroblast cell line or prostate carcinoma cell line (LNCaP) Culture Synchrony: Go or proliferation Computed

ORGANISM(S): Homo sapiens

SUBMITTER: Paul-Martin Holterhus

PROVIDER: E-GEOD-4453 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Cell-line and tissue-specific signatures of androgen receptor-coregulator transcription.

Bebermeier Jan-Hendrik JH Brooks James D JD DePrimo Samuel E SE Werner Ralf R Deppe Uta U Demeter Janos J Hiort Olaf O Holterhus Paul-Martin PM

Journal of molecular medicine (Berlin, Germany) 20060824 11

Normal genital skin fibroblasts (GSF) and the human prostate carcinoma cell line LNCaP have been used widely as cell culture models of genital origin to study androgen receptor (AR) signaling. We demonstrate that LNCaP shows a reproducible response to androgens as assessed using cDNA-microarrays representing approximately 32,000 unique human genes, whereas several independent GSF strains are virtually unresponsive. We show that LNCaP cells express markedly higher AR protein levels likely contrib ...[more]

PMID: 16932916

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Dataset Information

Androgen receptor coregulators baseline comparisons

Publications

Cell-line and tissue-specific signatures of androgen receptor-coregulator transcription.

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