Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Transcription profiling of Arabidopsis roots from slim1 and the parental line grown +/-S condition

ABSTRACT: Plants utilize soil sulfate for production of sulfur-containing amino acids that serve as essential dietary sulfur sources for animals. Despite the global nutritional significance of this fundamental metabolic process in nature, transcription factors regulating the plant sulfur assimilation pathways have never been discovered. We isolated sulfur limitation1 (slim1) mutants from Arabidopsis, showing abnormally low expression of SULTR1;2 sulfate transporter, by screening responsiveness of SULTR1;2 promoter-GFP, as an indicator, to sulfur limitation. SLIM1 encoded an EIL-family transcription factor, EIL3. To clarify the siganificance of SLIM1 function in sulfur responsive gene expression, we analyzed the transcriptome profiles in slim1-1, slim1-2 and the parental line under +S and -S conditions. Experiment Overall Design: PSULTR1;2-GFP, slim1-1 and slim1-2 were vertically grown on the +S/-S (S1500/S15) agar medium. Root tissues of 10-day-old plants were used for RNA extraction and hybridization on Affymetrix microarrays. All conditions were duplicated.

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Akiko Maruyama-Nakashita

PROVIDER: E-GEOD-4455 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Arabidopsis SLIM1 is a central transcriptional regulator of plant sulfur response and metabolism.

Maruyama-Nakashita Akiko A Nakamura Yumiko Y Tohge Takayuki T Saito Kazuki K Takahashi Hideki H

The Plant cell 20061117 11

Sulfur is an essential macronutrient required for plant growth. To identify key transcription factors regulating the sulfur assimilatory pathway, we screened Arabidopsis thaliana mutants using a fluorescent reporter gene construct consisting of the sulfur limitation-responsive promoter of the SULTR1;2 sulfate transporter and green fluorescent protein as a background indicator for monitoring plant sulfur responses. The isolated mutant, sulfur limitation1 (slim1), was unable to induce SULTR1;2 tra ...[more]

PMID: 17114350

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Dataset Information

Transcription profiling of Arabidopsis roots from slim1 and the parental line grown +/-S condition

Publications

Arabidopsis SLIM1 is a central transcriptional regulator of plant sulfur response and metabolism.

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