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Posterior Second Heart Field (pSHF) transcriptomes of wild-type and Shh mutant mouse embryos

ABSTRACT: To understand Sonic hedgehog homolog (Shh)-mediated molecular networks in the posterior second heart field (pSHF), we have employed whole genome microarray expression profiling as a discovery platform to identify genes with Shh-dependent expressional changes. We microdissected the pSHF from E9.5 embryos and compared the Shh mutant samples with than of wild-type using Agilent 4x44k Mouse Whole Genome Arrays (n = 4 WT pools and 4 Shh - /- pools). Microdissected pSHF from E9.5 mouse embryos was molecularly verified by real-time PCR. Shh mutant embryos were compared with wild-type embryos. Four independnet pools of RNAs from each biological group were measured on 1-color Agilent Mouse Whole Genome Arrays (n = 3 WT pools and 4 Shh -/- pools).

ORGANISM(S): Mus musculus

SUBMITTER: Xinan Yang

PROVIDER: E-GEOD-44754 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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