Ontology highlight
ABSTRACT:
ORGANISM(S): Tetrahymena thermophila
SUBMITTER: Wei Miao
PROVIDER: E-GEOD-44990 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
Genes & development 20130724 15
Replication of nuclear DNA occurs in the context of chromatin and is influenced by histone modifications. In the ciliate Tetrahymena thermophila, we identified TXR1, encoding a histone methyltransferase. TXR1 deletion resulted in severe DNA replication stress, manifested by the accumulation of ssDNA, production of aberrant replication intermediates, and activation of robust DNA damage responses. Paired-end Illumina sequencing of ssDNA revealed intergenic regions, including replication origins, a ...[more]