Unknown,Transcriptomics,Genomics,Proteomics

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High risk HPV infection effect on expression profile mediated by tumor necrosis factor

ABSTRACT: Previous studies have shown that normal and HPV immortalized keratinocytes are sensitive to TNF anti-proliferative effect. Conversely, HPV18-immortalized keratinocytes are resistant to the cytostatic effect mediated by this cytokine. In this study we have compared gene expression patterns in primary cultures of human foreskin epidermal keratinocytes (PHK or NHFK) and HPV16 (HF698) and HPV18(HF18Nco)-immortalized cell lines, and profile the transcriptional changes 3 and 60 hours after TNF treatment. Keywords: global expression profile, microarray, HPV, TNF In order to determine the effects of HPV infection and TNF treatment on global gene expression, were performed 2 independent experiment for each cell line, including the two periods of treatment with TNF. Furthermore, were performed 2 experiments for each control plate containing the non-treated cells.

ORGANISM(S): Homo sapiens

SUBMITTER: Lara Termini

PROVIDER: E-GEOD-4524 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Characterization of global transcription profile of normal and HPV-immortalized keratinocytes and their response to TNF treatment.

Termini Lara L Boccardo Enrique E Esteves Gustavo H GH Hirata Roberto R Martins Waleska K WK Colo Anna Estela L AE Neves E Jordão EJ Villa Luisa Lina LL Reis Luiz Fl LF

BMC medical genomics 20080627

<h4>Background</h4>Persistent infection by high risk HPV types (e.g. HPV-16, -18, -31, and -45) is the main risk factor for development of cervical intraepithelial neoplasia and cervical cancer. Tumor necrosis factor (TNF) is a key mediator of epithelial cell inflammatory response and exerts a potent cytostatic effect on normal or HPV16, but not on HPV18 immortalized keratinocytes. Moreover, several cervical carcinoma-derived cell lines are resistant to TNF anti-proliferative effect suggesting t ...[more]

PMID: 18588690

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Dataset Information

High risk HPV infection effect on expression profile mediated by tumor necrosis factor

Publications

Characterization of global transcription profile of normal and HPV-immortalized keratinocytes and their response to TNF treatment.

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