Transcription profiling of mouse primary visual cortext from animals treated with monocular deprivation (MD), dark rearing and controls reveals binding protein of Insulin-like Growth Factor 1 is highly upregulated after MD

ABSTRACT: Two key paradigms for examining activity-dependent development of primary visual cortex (V1) involve either reduction of activity in both eyes via dark-rearing (DR) or imbalance of activity between the two eyes via monocular deprivation (MD).,Combining DNA microarray analysis with computational approaches, RT-PCR, immunohistochemistry and physiological imaging, we find that DR leads to (i) upregulation of genes subserving synaptic transmission and electrical activity, consistent with a coordinated response of cortical neurons to reduction of visual drive, and (ii) downregulation of parvalbumin, implicating parvalbumin-expressing neurons as underlying the delay in cortical maturation after DR. MD partially activates homeostatic mechanisms but differentially upregulates gene systems related to growth factors and neuronal degeneration, consistent with reorganization of connections after MD. A binding protein of Insulin-like Growth Factor 1 is highly upregulated after MD, and exogenous application of IGF1 prevents the physiological effects of MD on ocular dominance plasticity examined in vivo.

ORGANISM(S): Mus musculus

SUBMITTER: gabriel kreiman 

PROVIDER: E-GEOD-4537 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress