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Expression data from mouse primary visual cortex under different deprivation conditions

ABSTRACT: Two key paradigms for examining activity-dependent development of primary visual cortex (V1) involve either reduction of activity in both eyes via dark-rearing (DR) or imbalance of activity between the two eyes via monocular deprivation (MD). Combining DNA microarray analysis with computational approaches, RT-PCR, immunohistochemistry and physiological imaging, we find that DR leads to (i) upregulation of genes subserving synaptic transmission and electrical activity, consistent with a coordinated response of cortical neurons to reduction of visual drive, and (ii) downregulation of parvalbumin, implicating parvalbumin-expressing neurons as underlying the delay in cortical maturation after DR. MD partially activates homeostatic mechanisms but differentially upregulates gene systems related to growth factors and neuronal degeneration, consistent with reorganization of connections after MD. A binding protein of Insulin-like Growth Factor 1 is highly upregulated after MD, and exogenous application of IGF1 prevents the physiological effects of MD on ocular dominance plasticity examined in vivo. Keywords: multiple comparisons

ORGANISM(S): Mus musculus

PROVIDER: GSE4537 | GEO | 2006/04/30

SECONDARY ACCESSION(S): PRJNA94463

REPOSITORIES: GEO

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Transcription profiling of mouse primary visual cortext from animals treated with monocular deprivation (MD), dark rearing and controls reveals binding protein of Insulin-like Growth Factor 1 is highly upregulated after MD

Project description:Two key paradigms for examining activity-dependent development of primary visual cortex (V1) involve either reduction of activity in both eyes via dark-rearing (DR) or imbalance of activity between the two eyes via monocular deprivation (MD).,Combining DNA microarray analysis with computational approaches, RT-PCR, immunohistochemistry and physiological imaging, we find that DR leads to (i) upregulation of genes subserving synaptic transmission and electrical activity, consistent with a coordinated response of cortical neurons to reduction of visual drive, and (ii) downregulation of parvalbumin, implicating parvalbumin-expressing neurons as underlying the delay in cortical maturation after DR. MD partially activates homeostatic mechanisms but differentially upregulates gene systems related to growth factors and neuronal degeneration, consistent with reorganization of connections after MD. A binding protein of Insulin-like Growth Factor 1 is highly upregulated after MD, and exogenous application of IGF1 prevents the physiological effects of MD on ocular dominance plasticity examined in vivo.

2007-07-07 | E-GEOD-4537 | biostudies-arrayexpress

Changes in miRNA expression in mouse primary visual cortex following visual deprivation

2011-09-04 | GSE31536 | GEO

Changes in miRNA expression in mouse primary visual cortex following visual deprivation

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2011-09-03 | E-GEOD-31536 | biostudies-arrayexpress

Transcription profiling of mouse visual cortex from wild type animals sacrificed at P18 after mononuclear enucleation

Project description:Wild-type (C57Bl6) mice underwent monocular enucleation at P14 and were sacrificed 4 days later at P18. Bilateral (non-deprived and deprived) visual cortex was dissected from 1-mm thick coronal sections, and total RNA was extracted from the tissue lysate using Trizol (Gibco-BRL). Experiment Overall Design: Gene expression in pooled samples from 5 non-deprived (ipsilateral to monocular enucleation) and 5 deprived (contralateral to monocular enucleation) visual hemispheres was compared.

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Transcription profiling of mouse visual cortex from P46 mouse non-deprived vs. deprived

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2008-06-13 | E-GEOD-4267 | biostudies-arrayexpress

Monocular Visual Deprivation in Macaque Monkeys: A Profile in the Gene Expression of the LGN by LCM

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2008-12-01 | GSE9795 | GEO

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