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Gene expression profiles of individual blastomeres of early Ciona embryos

ABSTRACT: We examined gene expression profiles of individual blastomeres of early Ciona embryos using microarrays. We examined gene expression profiles of individual blastomeres of the 8-cell, 16-cell and 32-cell embryos by dissecting with a fine glass needle. We also examined gene expression profiles of unfertilized eggs and embryos from the 1- to 32-cell stages.

ORGANISM(S): Ciona intestinalis

SUBMITTER: Yutaka Satou

PROVIDER: E-GEOD-45548 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Transcriptome dynamics in early embryos of the ascidian, Ciona intestinalis.

Matsuoka Terumi T Ikeda Tatsuro T Fujimaki Kotaro K Satou Yutaka Y

Developmental biology 20131009 2

Maternally provided mRNAs and proteins direct early development and activate the zygotic genome. Using microarrays, we examined the dynamics of transcriptomes during the early development of a basal chordate, Ciona intestinalis. Microarray analysis of unfertilized eggs, as well as 8-, and 16- and 32-cell embryos revealed that nearly half of the genes encoded in the genome were expressed maternally, and that approximately only one-fourth of these genes were expressed at similar levels among eggs ...[more]

PMID: 24120375

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