Ontology highlight
ABSTRACT:
ORGANISM(S): Homo sapiens
SUBMITTER: Jeffrey Parvin
PROVIDER: E-GEOD-45771 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
Arora Mansi M Zhang Jie J Heine George F GF Ozer Gulcin G Liu Hui-wen HW Huang Kun K Parvin Jeffrey D JD
Nucleic acids research 20120831 20
We analyzed modification of chromatin by ubiquitination in human cells and whether this mark changes through the cell cycle. HeLa cells were synchronized at different stages and regions of the genome with ubiquitinated chromatin were identified by affinity purification coupled with next-generation sequencing. During interphase, ubiquitin marked the chromatin on the transcribed regions of ∼70% of highly active genes and deposition of this mark was sensitive to transcriptional inhibition. Promoter ...[more]