Proteomics

Dataset Information

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RNF8 mediates NONO degradation following UV-induced DNA damage to properly terminate ATR-CHK1 checkpoint signaling


ABSTRACT: RNF8 is a key E3 ubiquitin ligase functioning in both DSB- and UV-induced DDR, identification of additional substrates will help further elucidate its role in DNA damage signaling. To identify substrates of ubiquitin ligases, we have recently devised a method based on proximity-dependent biotin labeling. In this method, an E3 ubiquitin ligase of interest is expressed as a fusion to Escherichia coli biotin ligase BirA together with a biotin acceptor peptide (AP)-tagged ubiquitin. The BirA-directed biotin labeling of AP depends on the proximity of the two fusion proteins in the cell, which leads to preferential labeling of ubiquitinated E3 substrates. In this study, we applied this procedure to RNF8 and identified its substrates.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Permanent Cell Line Cell, Hela Cell

DISEASE(S): Cervical Adenocarcinoma

SUBMITTER: Rakesh Deshar  

LAB HEAD: Jong-Bok Yoon

PROVIDER: PXD006156 | Pride | 2018-11-07

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
140912_RNF8_KGG_RK.mgf Mgf
140912_RNF8_KGG_RK.pride.mgf.gz Mgf
NONO-KGG-MGF.mgf Mgf
NONO-KGG-MGF.pride.mgf.gz Mgf
NONO-KGG-Mziden.mzid.gz Mzid
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Publications

RNF8 mediates NONO degradation following UV-induced DNA damage to properly terminate ATR-CHK1 checkpoint signaling.

Deshar Rakesh R   Yoo Wonjin W   Cho Eun-Bee EB   Kim Sungjoo S   Yoon Jong-Bok JB  

Nucleic acids research 20190101 2


RNF8 plays a critical role in DNA damage response (DDR) to initiate ubiquitination-dependent signaling. To better characterize the role of RNF8 in UV-induced DDR, we searched for novel substrates of RNF8 and identified NONO as one intriguing substrate. We found that: (i) RNF8 ubiquitinates NONO and (ii) UV radiation triggers NONO ubiquitination and its subsequent degradation. Depletion of RNF8 inhibited UV-induced degradation of NONO, suggesting that RNF8 targets NONO for degradation in response  ...[more]

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