Unknown,Transcriptomics,Genomics,Proteomics

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Human cystatin D (CST5) locates in the nucleus at sites of active transcription and regulates gene expression

ABSTRACT: Cystatin D (CST5) is an inhibitor of several proteases of the cathepsin family that inhibits cell proliferation, migration and invasion of colon carcinoma cells. Some of these effects are unrelated to its antiprotease activity. Here, we use genome-wide expression microarrays to show that cystatin D regulates gene expression (including that of genes encoding transcription factors such as RUNX1, RUNX2, or MEF2C) in HCT116 cells. HCT116 colon adenocarcinoma cell lines stably expressing cystatin D (HCT116-CST5 clone 9 and clone 20), mock-transfected (HCT116-Mock) and un-transfected (HCT116-Untransfected) were generated as described (Ãlvarez-DÃaz et al., 2009, PUBMED:19662683) and genome-wide expression microarrays were obtained to achieve the gene expression profile induced by CST5.

ORGANISM(S): Homo sapiens

SUBMITTER: Javier De Las Rivas

PROVIDER: E-GEOD-45904 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Cystatin D locates in the nucleus at sites of active transcription and modulates gene and protein expression.

Ferrer-Mayorga Gemma G Alvarez-Díaz Silvia S Valle Noelia N De Las Rivas Javier J Mendes Marta M Barderas Rodrigo R Canals Francesc F Tapia Olga O Casal J Ignacio JI Lafarga Miguel M Muñoz Alberto A

The Journal of biological chemistry 20150913 44

Cystatin D is an inhibitor of lysosomal and secreted cysteine proteases. Strikingly, cystatin D has been found to inhibit proliferation, migration, and invasion of colon carcinoma cells indicating tumor suppressor activity that is unrelated to protease inhibition. Here, we demonstrate that a proportion of cystatin D locates within the cell nucleus at specific transcriptionally active chromatin sites. Consistently, transcriptomic analysis show that cystatin D alters gene expression, including tha ...[more]

PMID: 26364852

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Dataset Information

Human cystatin D (CST5) locates in the nucleus at sites of active transcription and regulates gene expression

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Cystatin D locates in the nucleus at sites of active transcription and modulates gene and protein expression.

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