Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcriptomic analysis of phosphatin effect-Phosphatin effect on Arabidopsis

ABSTRACT: cea11-02_phosphatin - transcriptomic analysis of phosphatin effect - Identification of transcriptomic modifications promoted by phosphatin (AC6) (a molecule mimicking Pi addition effects on Pi starved plants). - 40 ÂµM Phosphatin was added to MS (diluted 10 times) containing 20ÂµM phosphate medium and controls were grown on MS (diluted 10 times) containing 20ÂµM phosphate or supplemented with 500 ÂµM of Pi. For each ARN extraction their was 3 plates containing each 10 seedlings grown 13 days in vertical petri dishes. 6 dye-swap - treated vs untreated comparison

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Soubigou-Taconnat Ludivine

PROVIDER: E-GEOD-45937 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

atnsrs-study the role of AtNSRs proteins in the regulation of gene expression

Project description:blanc-08-01_2012_01_rnapaths_nsr - atnsrs - study the role of AtNSRs proteins in the regulation of gene expression - comparaison between Arabidopsis thaliana Col-0 and Atnsra/b double mutant treated or not with 24h 1ÂµM NAA 12 dye-swap - gene knock out

2012-10-01 | E-GEOD-39659 | biostudies-arrayexpress

Comparison after 4 and 52 h (Chloro-Sap)

Project description:cea12-02_chlorosap - comparison after 4 and 52h - To observe the effects of guanosine penta/tetraphosphate on gene expression - Plants of four different genotypes (43A10.6, 44B1.3, H1 P3.2 and Col-0) were grown for ten days on MS/2 plates with 1% sucrose under 80ÂµE light in long-day conditions (16 hours of light, 8 hours of dark). Plants were treated at 10:30 in the morning (Time 0; 10:30 is 3 and a half hours after the onset of the light period) by flooding with 30uM dexamethasone (in aqueous solution with 0,3 % DMSO and 0,033 % Silwet L-77) for 5 minutes . Samples were taken at 4 hrs and 52 hrs after T0. Aerial parts of the seedlings were rapidly harvested and frozen in liquid nitrogen. 9 dye-swap - normal vs transgenic comparaison,time course

2014-11-01 | E-GEOD-62656 | biostudies-arrayexpress

transcripomics on the arabidopsis root tip during phosphate starvation-Transcripomics on the Arabidopsis Root tip during phosphate starvation

Project description:The objective of this project is to identify genes that are expressed in the Arabidopsis thaliana root tip and that are early induced (or repressed) by phoshate deficiency. Seedlings were germinated and grown (for 6 days) on phosphate rich medium and transfered to either a phosphate poor medium (Pi- = 20uM Pi treatment) or a phosphate rich medium (Pi+ = 500uM, Pi = control). Fifteen and 60 minutes after transfer, the tip (~800um) of the primary root was cut under a dissecting microscope. About 100 root tips per condition were harvested and there mRNA was analysed with the use of microarrays. 6 dye-swap - time course,treated vs untreated comparison

2011-09-08 | E-GEOD-31135 | biostudies-arrayexpress

bHLH39 overexpression

Project description:au13-06_fit - bhlh39 overexpression - Analyse the responses to bHLH39 overexpression. bHLH39 is one of the transcription factors regulating iron homeostasis responses. - Six day-old seedlings (wild type and bHLH39 overexpressor) were grown on Hoagland medium. Full seedlings were harvested for analysis. 21 dye-swap - normal vs transgenic comparaison

2015-02-14 | E-GEOD-65934 | biostudies-arrayexpress

Response to salt stress of Raptor mutants

Project description:ra12-03_raptor-nacl - Characterization to raptor mutants (partner of the kinase TOR) and of their answer to the salt stress. - Sowing on metal grids on MS/2 medium with continuous light. Transfer after 12 days of culture for 3h to liquid MS/2 medium containing or not 200 mM NaCl. 7 dye-swap - gene knock out, treated vs untreated comparison

2013-07-01 | E-GEOD-47391 | biostudies-arrayexpress

Endogenous hairpins-ir71 targets : Gene profiling of Turnip Crinkle Virus (TCV) siRNA in mutants.

Project description:gnp06-03_microtrac Endogenous hairpins-ir71 targets What are the genes (including miRNA precursors) that are differentially regulated in a set of viral siRNA in diferent mutants? Lines IR71 KO nÂ°3 and Col-0 were grown on soil until bolting, Flower buds were then harvested and RNA extracted with Qiagen RNeasy Plant mini kit. RNA was stored at -20Â°C. 3 dye-swap - gene knock out

2012-12-31 | E-GEOD-34653 | biostudies-arrayexpress

rnapaths--3_02/2012-RNAPATHS-3

Project description:blanc-08-01_2012_01_rnapaths_03 - rnapaths--3_02/2012 - Identify the transcript overlap and specificity between the PTGS and decapping/exoribonuclease pathways b identifying transcripts that are significantly changed in double mutants versus single mutants, and transcripts that are commonly changed among the single and double mutants compared to WT. - Identify transcripts that are significantly changed in double mutants (L1 vcs sgs2) (xrn4-5/sgs3-11) versus their respective single mutants (L1 vcs and L1 sgs2) (xrn4-5 and sgs3-11) , and identify transcripts that are changed among the single and double mutants compared to WT (Col) reference or to mutant L1 reference. 20 dye-swap - genotype comparaison

2012-07-12 | E-GEOD-38903 | biostudies-arrayexpress

abaseed-ABA signaling in Arabidopsis seeds

Project description:ra14-04_nced - abaseed - Identification of abscisic acid (ABA) signaling factors and targets in Arabidopsis developing seeds by comparing ABA deficient and wild type genotypes. Transcriptome comparison of developing seeds harvested at 14 DAP from two ABA-deficient mutants (nced2 nced5 nced9 and nced2 nced5 nced6 nced9) and wild type (Columbia-0). 6 dye-swaps - gene knockout, genomic comparison.

2015-07-01 | E-GEOD-68088 | biostudies-arrayexpress

Effect of earthworms on Arabidopsis transcriptome

Project description:au07-05_groundworms - groundworms - Do earthworms affect plant growth through signal molecules? - Plants were grown in a sandy soil from the Centre de Recherche en Ecologie Expérimentale et Prédictive (CEREEP, France), in the presence or absence of earthworms. Soil was dried and sieved at 2 mm before to be put in the pots. There was 2 levels of treatment (presence or absence of earthworms in the soil), and 5 plants per treatments. Keywords: treated vs untreated comparison 4 dye-swap - CATMA arrays

2010-09-30 | E-GEOD-24393 | biostudies-arrayexpress

myb118 mutation; Identification of genes misregulated in developing seeds of Arabidopsis in response to the myb118 mutation.

Project description:ra12-06_myb118; myb118 mutation What are the genes over expressed or down regulated in seeds in response to a myb118 mutation? Plants of the different genotypes (wild-type, myb118 mutant, myb118 antisense lines named 0E3 and T16) were grown in a green house, their flower were tagged and seeds were harvested 10 days after anthesis. Seeds were extracted from the siliques, stored at -80Â°C prior to RNA extraction. 6 dye-swap - normal vs transgenic comparaison

2013-12-01 | E-GEOD-48180 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data