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Comparison between all runs of industrial serial repitching 1

ABSTRACT: The aim of this study was to identify differently expressed genes between all runs of four serial repitchings from a German brewery. In the brewery the number of successive runs varied from 17 to 20. A run describes the industrial fermentation of brewery wort. By the end of fermentation the yeast slurry is cropped. These cells are reused for inoculation of another cylindro-conical vessel (CCVs). Results from four loops are summarized in this study. The samples were taken from four serial repitchings of a German brewery (brewery A). Microarrays were hybridized in a loop approach.

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Frank Stahl

PROVIDER: E-GEOD-45943 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Sustainability of industrial yeast serial repitching practice studied by gene expression and correlation analysis.

Bühligen Franziska F Rüdinger Philipp P Fetzer Ingo I Stahl Frank F Scheper Thomas T Harms Hauke H Müller Susann S

Journal of biotechnology 20130921 4

Bottom-fermenting Saccharomyces pastorianus strains driving brewing fermentation processes are usually reused several times. It is still unclear, whether the number of successions may have an impact on cell physiology prompting consequences for brewing quality. In this study, fermentation performance of up to twenty consecutive runs in a brewery was investigated. For each run mRNA expression levels of cellular marker molecules, which are known to correlate with metabolism, hexose transport, agin ...[more]

PMID: 24060829

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