Unknown,Transcriptomics,Genomics,Proteomics

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Gene Expression Changes in the Development of the Soybean Seed-Early Maturation Stage


ABSTRACT: We investigated soybean seed development because (1) soybean seeds are a major source of food and fuel, (2) soybean seeds have been an excellent system for studying the basic processes controlling seed development for over three decades, and (3) new soybean genomic resources, including the sequence of the soybean genome and the gene expression profiles for all seed compartments, tissues, and cell types, can be used to gain new insights into the regulatory processes required for seed differentiation. We sequenced messenger RNA populations of specific soybean seed compartments, which will provide new insights into gene expression that are important for â??making a soybean seed.â?? Seventeen compartments of the Early Maturation stage of the soybean seeds were analyzed. Three to four biological replicates were collected for each compartment.

ORGANISM(S): Glycine max

SUBMITTER: Julie Pelletier 

PROVIDER: E-GEOD-46096 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Down-Regulating the Expression of 53 Soybean Transcription Factor Genes Uncovers a Role for SPEECHLESS in Initiating Stomatal Cell Lineages during Embryo Development.

Danzer John J   Mellott Eric E   Bui Anhthu Q AQ   Le Brandon H BH   Martin Patrick P   Hashimoto Meryl M   Perez-Lesher Jeanett J   Chen Min M   Pelletier Julie M JM   Somers David A DA   Goldberg Robert B RB   Harada John J JJ  

Plant physiology 20150511 3


We used an RNA interference screen to assay the function of 53 transcription factor messenger RNAs (mRNAs) that accumulate specifically within soybean (Glycine max) seed regions, subregions, and tissues during development. We show that basic helix-loop-helix (bHLH) transcription factor genes represented by Glyma04g41710 and its paralogs are required for the formation of stoma in leaves and stomatal precursor complexes in mature embryo cotyledons. Phylogenetic analysis indicates that these bHLH t  ...[more]

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