Genome-Wide Small RNA Profiling of a Soybean Seed Compartment-Early Maturation Stage
Ontology highlight
ABSTRACT:
ORGANISM(S): Glycine max
SUBMITTER: Julie Pelletier
PROVIDER: E-GEOD-57906 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
Similar Datasets
Project description:We performed small-RNA profiling of soybean seed regions and sub-regions, to gain new insights into the regulatory processes required for seed differentiation. Eight subregions of the Cotyledon stage of the soybean seeds were analyzed. Two biological replicates were collected for each subregions.
2014-05-22 | E-GEOD-57883 | biostudies-arrayexpress
Project description:We performed small-RNA profiling of soybean seed regions and sub-regions, to gain new insights into the regulatory processes required for seed differentiation. Four subregions of the Globular stage of the soybean seeds were analyzed. Two biological replicates were collected for each subregions.
2014-05-21 | E-GEOD-57845 | biostudies-arrayexpress
Project description:We performed small-RNA profiling of soybean seed regions and sub-regions, to gain new insights into the regulatory processes required for seed differentiation. Four subregions of the Heart stage of the soybean seeds were analyzed. Two biological replicates were collected for each subregions.
2014-05-22 | E-GEOD-57874 | biostudies-arrayexpress
Project description:We investigated soybean seed development because (1) soybean seeds are a major source of food and fuel, (2) soybean seeds have been an excellent system for studying the basic processes controlling seed development for over three decades, and (3) new soybean genomic resources, including the sequence of the soybean genome and the gene expression profiles for all seed compartments, tissues, and cell types, can be used to gain new insights into the regulatory processes required for seed differentiation. We sequenced messenger RNA populations of specific soybean seed compartments, which will provide new insights into gene expression that are important for M-bM-^@M-^\making a soybean seed.M-bM-^@M-^] Ten compartments of the Cotyledon stage of the soybean seeds were analyzed. Three biological replicates were collected for each compartment.
2014-05-14 | E-GEOD-57606 | biostudies-arrayexpress
Project description:We investigated soybean seed development because (1) soybean seeds are a major source of food and fuel, (2) soybean seeds have been an excellent system for studying the basic processes controlling seed development for over three decades, and (3) new soybean genomic resources, including the sequence of the soybean genome and the gene expression profiles for all seed compartments, tissues, and cell types, can be used to gain new insights into the regulatory processes required for seed differentiation. We sequenced messenger RNA populations of specific soybean seed compartments, which will provide new insights into gene expression that are important for â??making a soybean seed.â?? Seventeen compartments of the Early Maturation stage of the soybean seeds were analyzed. Three to four biological replicates were collected for each compartment.
2013-04-17 | E-GEOD-46096 | biostudies-arrayexpress
Project description:We investigated soybean seed development because (1) soybean seeds are a major source of food and fuel, (2) soybean seeds have been an excellent system for studying the basic processes controlling seed development for over three decades, and (3) new soybean genomic resources, including the sequence of the soybean genome and the gene expression profiles for all seed compartments, tissues, and cell types, can be used to gain new insights into the regulatory processes required for seed differentiation. We sequenced messenger RNA populations of specific soybean seed compartments, which will provide new insights into gene expression that are important for M-bM-^@M-^\making a soybean seed.M-bM-^@M-^] Eight compartments of the globular stage of the soybean seeds were analyzed. Three or four biological replicates were collected for each compartment.
2014-05-07 | E-GEOD-57349 | biostudies-arrayexpress
Project description:We investigated soybean seed development because (1) soybean seeds are a major source of food and fuel, (2) soybean seeds have been an excellent system for studying the basic processes controlling seed development for over three decades, and (3) new soybean genomic resources, including the sequence of the soybean genome and the gene expression profiles for all seed compartments, tissues, and cell types, can be used to gain new insights into the regulatory processes required for seed differentiation. We sequenced messenger RNA populations of specific soybean seed compartments, which will provide new insights into gene expression that are important for M-bM-^@M-^\making a soybean seed.M-bM-^@M-^] Eight compartments of the heart stage of the soybean seeds were analyzed. Three biological replicates were collected for each compartment.
2014-05-07 | E-GEOD-57350 | biostudies-arrayexpress
Project description:This experiment combines four RNA-Seq datasets of soybean seeds at the following developmental stages: 1) seed globular stage, E-GEOD-57349 (https://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-57349/); 2) seed heart stage, E-GEOD-57350 (https://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-57350/); 3) seed cotyledon stage, E-GEOD-57606 (https://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-57606/) and 4) seed early maturation stage, E-GEOD-46096 (https://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-46096/). Authors investigated soybean seed development because (1) soybean seeds are a major source of food and fuel, (2) soybean seeds have been an excellent system for studying the basic processes controlling seed development for over three decades, and (3) new soybean genomic resources, including the sequence of the soybean genome and the gene expression profiles for all seed compartments, tissues, and cell types, can be used to gain new insights into the regulatory processes required for seed differentiation. Authors sequenced messenger RNA populations of specific soybean seed compartments to provide new insights into gene expression that are important for âmaking a soybean seed.â
2014-05-07 | E-MTAB-4045 | biostudies-arrayexpress
Project description:We report the genome-wide small RNA of soybean early maturation seed coat parenchyma compartment soybean early maturation seeds using Illumina high-throughput sequencing technology. Illumina sequencing of small RNA from early maturation seed coat parenchyma compartment and early-maturation stage whole seeds
2011-12-22 | E-GEOD-34638 | biostudies-arrayexpress
Project description:Developing effective model systems to evaluate new potential chemotherapeutic reagents is critical. Three-dimensional cell cultures, such as spheroids, aid in bridging the gap between commonly used monolayer cell cultures and significantly more complex and expensive animal models. Spheroid model systems contain pathophysiological and chemical gradients similar to an in vivo tumor, which ultimately form distinct cellular subpopulations. In the spatial SILAC model, labels are pulsed into the spheroid at discrete time windows during development. These media pulses result in labeled proteins in distinct regions of the spheroid, which allows for tracing of quantitative proteomic changes to be correlated to different cellular subregions. In this study, we use the Spatial SILAC model to evaluate the proteomic response of a colon carcinoma spheroid to the multikinase inhibitor Regorafenib. We determined regorafenib to be an effective kinase inhibitor which significantly altered whole spheroid proliferation and resulted in increased apoptosis when the signal was averaged for all cells in the culture. However, when regorafenib treatment is more closely examined among the cellular subpopulations, drastic differences are observed for how the drug impacted the proteome of the necrotic spheroid core and the proliferating outer regions. Whole spheroid and outer region analysis shows that regorafenib treatment inhibited critical pathways such as mTOR signaling, ERK/MAPK signaling, and colorectal cancer metastasis signaling. However, analysis of the core shows that regorafenib had an entirely different effect, including upregulation of MAPK1 and KRAS, possibly indicating drug resistance within these late apoptotic cells. Ultimately, these combinatory studies can be used to further understanding of drug metabolism is different cellular subpopulations and provide valuable information to ultimately improve the accuracy of therapeutic testing.
2024-08-09 | PXD038958 | Pride