Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of galactose-grown S. cerevisiae cells exposed to lithium chloride


ABSTRACT: Wild type strain CEN.PK113-7D was grown in an aerobic batch cultivation with a start concentration of 20 g/L galactose. During exponential growth at a biomass concentration of 3 g dry weight/L LiCl was added to a concentration of 10 mM. Just before addition of LiCl (time 0) and 20, 40, 60 and 140 minutes after addition of LiCl samples were taken for transcription analysis. Lithium inhibits phosphoglucomutase whereby both galactose uptake and growth is strongly affected.

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Christoffer Bro 

PROVIDER: E-GEOD-461 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Transcriptional, proteomic, and metabolic responses to lithium in galactose-grown yeast cells.

Bro Christoffer C   Regenberg Birgitte B   Lagniel Gilles G   Labarre Jean J   Montero-Lomelí Mónica M   Nielsen Jens J  

The Journal of biological chemistry 20030605 34


Lithium is highly toxic to yeast when grown in galactose medium mainly because phosphoglucomutase, a key enzyme of galactose metabolism, is inhibited. We studied the global protein and gene expression profiles of Saccharomyces cerevisiae grown in galactose in different time intervals after addition of lithium. These results were related to physiological studies where both secreted and intracellular metabolites were determined. Microarray analysis showed that 664 open reading frames were down-reg  ...[more]

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