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Impairment of Muscle Regeneration in Muscleblind-like 3 Isoform Knockout Mice

ABSTRACT: We have performed an RNA-seq experiment to identify expression and alternative splicing differences between WT and Mbnl3 isoform knockout mice E15 forelimbs. We have also identified and characterized transcriptome wide Mbnl3-binding sites in C2C12 cells and E15 forelimbs. 6 total samples were analyzed: E15 forelimbs from 3 WT and 3 MBNL3 M-NM-^TE2/M-NM-^TE2 female mice (15dpc). For HITS-CLIP, 3 samples each of C2C12 cells, WT E15 forelimbs and Mbnl3 ?E2 forelimbs were analyzed for Mbnl3 binding site analysis.

ORGANISM(S): Mus musculus

SUBMITTER: Ranjan Batra

PROVIDER: E-GEOD-46207 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Progressive impairment of muscle regeneration in muscleblind-like 3 isoform knockout mice.

Poulos Michael G MG Batra Ranjan R Li Moyi M Yuan Yuan Y Zhang Chaolin C Darnell Robert B RB Swanson Maurice S MS

Human molecular genetics 20130508 17

The muscleblind-like (MBNL) genes encode alternative splicing factors that are essential for the postnatal development of multiple tissues, and the inhibition of MBNL activity by toxic C(C)UG repeat RNAs is a major pathogenic feature of the neuromuscular disease myotonic dystrophy. While MBNL1 controls fetal-to-adult splicing transitions in muscle and MBNL2 serves a similar role in the brain, the function of MBNL3 in vivo is unknown. Here, we report that mouse Mbnl3, which encodes protein isofor ...[more]

PMID: 23660517

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