Unknown,Transcriptomics,Genomics,Proteomics

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Isolation and Characterization of a Novel Population of Pluripotent Stem Cells Derived from Human Adipose Tissue

ABSTRACT: Transcriptional comparison of two subpopulations of stem cells isolated from lipoaspirated human adipose tissue, profiling Adipose Stem Cells (ASCs) and Multilineage Differentiating Stress-Enduring Adipose Tissue cells (MUSE-ATs). Experiments focused in the isolation and characterization of a new population of adipose tissue (AT) derived pluripotent stem cells, termed MUSE-ATs, which are isolated using severe cellular stress conditions, including long-term exposure to the proteolytic enzyme collagenase, serum deprivation, low temperatures and hypoxia. Under these conditions, a homogenous, highly purified population of Muse-AT cells is isolated without the utilization of cell sorting methods. The goal of this experiment was to characterize MUSE-ATs, as well as compare them to the endogenous subpopulation of ASCs for reference. Single condition experiment, MUSE-ATs vs. ASCs. Biological replicates: 3 MUSE-ATs replicates, 3 ASCs replicates for reference.

ORGANISM(S): Homo sapiens

SUBMITTER: Saleh Heneidi

PROVIDER: E-GEOD-46353 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Isolation and Characterization of a Novel Population of Pluripotent Stem Cells Derived from Human Adipose Tissue

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