Ontology highlight
ABSTRACT:
ORGANISM(S): Mus musculus
SUBMITTER: Courtney Onodera
PROVIDER: E-GEOD-46396 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
Sachs Michael M Onodera Courtney C Blaschke Kathryn K Ebata Kevin T KT Song Jun S JS Ramalho-Santos Miguel M
Cell reports 20130530 6
Developmental regulatory genes have both activating (H3K4me3) and repressive (H3K27me3) histone modifications in embryonic stem cells (ESCs). This bivalent configuration is thought to maintain lineage commitment programs in a poised state. However, establishing physiological relevance has been complicated by the high number of cells required for chromatin immunoprecipitation (ChIP). We developed a low-cell-number chromatin immunoprecipitation (low-cell ChIP) protocol to investigate the chromatin ...[more]