Unknown,Transcriptomics,Genomics,Proteomics

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Bivalent Chromatin Marks Developmental Regulatory Genes in the Mouse Embryonic Germline in Vivo


ABSTRACT: We developed a ChIP protocol for the analysis of histone marks using less than 10,000 cells per IP, and used it to investigate the chromatin state of E11.5 mouse primordial germ cells (PGCs). A genome-wide ChIP-Seq analysis of E11.5 PGCs revealed a distribution of H3K4me3/H3K27me3 bivalent domains highly enriched for developmental regulatory genes. H3K4me3 and H3K27me3 ChIP-Seq from mouse E11.5 primordial germ cells.

ORGANISM(S): Mus musculus

SUBMITTER: Courtney Onodera 

PROVIDER: E-GEOD-46396 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Bivalent chromatin marks developmental regulatory genes in the mouse embryonic germline in vivo.

Sachs Michael M   Onodera Courtney C   Blaschke Kathryn K   Ebata Kevin T KT   Song Jun S JS   Ramalho-Santos Miguel M  

Cell reports 20130530 6


Developmental regulatory genes have both activating (H3K4me3) and repressive (H3K27me3) histone modifications in embryonic stem cells (ESCs). This bivalent configuration is thought to maintain lineage commitment programs in a poised state. However, establishing physiological relevance has been complicated by the high number of cells required for chromatin immunoprecipitation (ChIP). We developed a low-cell-number chromatin immunoprecipitation (low-cell ChIP) protocol to investigate the chromatin  ...[more]

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