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Expression profiling of E. coli rpoS knock-out strain during stationary phase in M9 minimal media with 0.2% glucose

ABSTRACT: Investigation of whole genome gene expression level in E. coli rpoS knock-out strain grown up to stationary phase in M9 minimal media supplemented with 0.2% glucose E. coli rpoS deletion mutant grown up to OD600nm 1.5 (stationary phase) in M9 minimal media supplemented with 0.2% glucose. The high-density oligonucleotide tiling arrays used were consisted of 371,034 oligonucleotide. Data for wild type controls are GSM389302, GSM389303, and GSM389304.

ORGANISM(S): Escherichia coli str. K-12 substr. MG1655

SUBMITTER: Donghyuk Kim

PROVIDER: E-GEOD-46543 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Genome-scale reconstruction of the sigma factor network in Escherichia coli: topology and functional states.

Cho Byung-Kwan BK Kim Donghyuk D Knight Eric M EM Zengler Karsten K Palsson Bernhard O BO

BMC biology 20140124

<h4>Background</h4>At the beginning of the transcription process, the RNA polymerase (RNAP) core enzyme requires a σ-factor to recognize the genomic location at which the process initiates. Although the crucial role of σ-factors has long been appreciated and characterized for many individual promoters, we do not yet have a genome-scale assessment of their function.<h4>Results</h4>Using multiple genome-scale measurements, we elucidated the network of σ-factor and promoter interactions in Escheric ...[more]

PMID: 24461193

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