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Transcription profiling of mouse osteoblasts under static vs simulated microgravity

ABSTRACT: The below table includes a smaller list of data that was analyzed by dChip and filtered by pvalue such that a file with about 4600 genes was obtained, which allowed for ease of use from 40,000 genes. Experiment Overall Design: The total RNA was extracted from 2T3 pre-osteoblast cells exposed to static or simulated microgravity (Rotating Wall Vessel) conditions. The RNA was then sent to Affymetrix microarray core facility at Baylor College of Medicine (Houston, TX) for microarray analysis.

ORGANISM(S): Mus musculus

SUBMITTER: Mamta Patel

PROVIDER: E-GEOD-4658 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Identification of mechanosensitive genes in osteoblasts by comparative microarray studies using the rotating wall vessel and the random positioning machine.

Patel Mamta J MJ Liu Wenbin W Sykes Michelle C MC Ward Nancy E NE Risin Semyon A SA Risin Diana D Jo Hanjoong H

Journal of cellular biochemistry 20070601 3

Weightlessness or microgravity of spaceflight induces bone loss due in part to decreased bone formation by unknown mechanisms. Due to difficulty in performing experiments in space, several ground-based simulators such as the Rotating Wall Vessel (RWV) and Random Positioning Machine (RPM) have become critical venues to continue studying space biology. However, these simulators have not been systematically compared to each other or to mechanical stimulating models. Here, we hypothesized that expos ...[more]

PMID: 17243119

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static vs simulated microgravity

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