Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Identification of DNA methylation changes in monocytes to osteoclast differentiation

ABSTRACT: Conversion of monocytes to osteoclasts is a unique terminal differentiation process within the hematopoietic system involving differentiation and massive cell fusion. Here we focused on DNA methylation changes during osteoclastogenesis. Hypermethylation and hypomethylation changes took place in several thousand genes, including all relevant functional categories in osteoclast differentiation and function. Comparison between the DNA methylation levels of CD14+ monocytes and derived osteoclast from 3 female donors. Bisulphite converted DNA from the 6 samples was hybridised to the Illumina Infinium 450k Human Methylation Beadchip

ORGANISM(S): Homo sapiens

SUBMITTER: Esteban Ballestar

PROVIDER: E-GEOD-46648 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

PU.1 target genes undergo Tet2-coupled demethylation and DNMT3b-mediated methylation in monocyte-to-osteoclast differentiation.

de la Rica Lorenzo L Rodríguez-Ubreva Javier J García Mireia M Islam Abul B M M K AB Urquiza José M JM Hernando Henar H Christensen Jesper J Helin Kristian K Gómez-Vaquero Carmen C Ballestar Esteban E

Genome biology 20130101 9

<h4>Background</h4>DNA methylation is a key epigenetic mechanism for driving and stabilizing cell-fate decisions. Local deposition and removal of DNA methylation are tightly coupled with transcription factor binding, although the relationship varies with the specific differentiation process. Conversion of monocytes to osteoclasts is a unique terminal differentiation process within the hematopoietic system. This differentiation model is relevant to autoimmune disease and cancer, and there is abun ...[more]

PMID: 24028770

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Dataset Information

Identification of DNA methylation changes in monocytes to osteoclast differentiation

Publications

PU.1 target genes undergo Tet2-coupled demethylation and DNMT3b-mediated methylation in monocyte-to-osteoclast differentiation.

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