Proteomics

Dataset Information

0

Human osteoclast derived from SAPHO PBMC


ABSTRACT: Inducing the PBMCs from SAPHO patients differentiation into osteoclasts while stimulating with serum exosomes. Compared with exosomes from normal volunteer, we found that about 2/3 of patients’ osteoclast differentiation were inhibited in response to exosomes from SAPHO patients. We present a TMT-labeled quantitative proteomics analysis of the osteoclasts which respond differently to different exosomes.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Wei Ge  

LAB HEAD: Wei Ge

PROVIDER: PXD012218 | Pride | 2021-09-08

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
MS181501SAPHO-PBMC.msf Msf
MS181501SAPHO-PBMC.pdResult Other
MS181501SAPHO-PBMC.pep.xml Pepxml
MS181501SAPHO-PBMC.xlsx Xlsx
MS181501_1.raw Raw
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Publications

Serum-derived extracellular vesicles inhibit osteoclastogenesis in active-phase patients with SAPHO syndrome.

Gao Yanpan Y   Chen Yanyu Y   Wang Lun L   Li Chen C   Ge Wei W  

Therapeutic advances in musculoskeletal disease 20210416


<h4>Objective</h4>Synovitis, acne, pustulosis, hyperostosis, and osteitis (SAPHO) syndrome is a rare chronic inflammatory disorder and the underlying pathogenesis is unclear. In this study, 88 SAPHO patients and 118 healthy controls were recruited to investigate the role of serum-derived extracellular vesicles (SEVs) in SAPHO syndrome.<h4>Methods</h4>Quantitative proteomics was applied for SEVs proteome identification, and ELISA and Western blotting was performed to verify the results of mass sp  ...[more]

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