Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of human skeletal muscle samples from geriatric and pediatric individuals


ABSTRACT: There is a consistent variation in the response of different skeletal muscle groups to mutations in genes known to cause muscular dystrophy, yet these muscles appear histologically similar. To better understand these phenotypic differences, we analyzed gene expression patterns in control muscle specimens obtained from four sites at autopsy: deltoid, quadriceps, gastrocnemius, and tibialis anterior (TA). A total of 35 muscle samples from nine individuals (four pediatric and five geriatric) were studied. Factors potentially influencing gene expression in the different samples included individuality, age, muscle type, gender, cause of death, postmortem interval, and ethnicity. The first three factors, in decreasing order, were found to have a significant impact on the stratification of muscle specimens. A novel analytic method, using a second round of normalization, was used to elicit differences between muscle types. This approach may be extended to a broader survey, potentially elucidating a molecular classification of the skeletal muscles.

ORGANISM(S): Homo sapiens

SUBMITTER: Peter Kang 

PROVIDER: E-GEOD-4667 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Variations in gene expression among different types of human skeletal muscle.

Kang Peter B PB   Kho Alvin T AT   Sanoudou Despina D   Haslett Judith N JN   Dow Chad P CP   Han Mei M   Blasko Jessica M JM   Lidov Hart G W HG   Beggs Alan H AH   Kunkel Louis M LM  

Muscle & nerve 20051001 4


There is a consistent variation in the response of different skeletal muscle groups to mutations in genes known to cause muscular dystrophy, yet these muscles appear histologically similar. To better understand these phenotypic differences, we analyzed gene expression patterns in control muscle specimens obtained from four sites at autopsy: deltoid, quadriceps, gastrocnemius, and tibialis anterior (TA). A total of 35 muscle samples from nine individuals (four pediatric and five geriatric) were s  ...[more]

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