Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

Expression data from E13.5 Fetal Liver LSK Hes1-GFP positive and E13.5 Fetal Liver LSK Hes1-GFP negative

ABSTRACT: Notch signaling defines a conserved, fundamental pathway, responsible for determination in metazoan development and is widely recognized as an essential component of lineage specific differentiation and stem cell self-renewal in many tissues including the hematopoietic system. Until recently, the majority of studies in the hematopoietic system focused on Notch signaling in lymphocyte differentiation and knowledge of individual Notch receptor roles in early hematopoiesis has been limited due to a paucity of genetic tools available To fate-map Notch receptor expression and pathway activity in the hematopoietic system we used tamoxifen-inducible CreER knock-in mice for individual Notch receptors in combination to a novel Notch reporter strain (Hes1GFP) and a conditional gain of function allele of Notch2 receptor (Rosa-lsl-ICN2). E13.5 Foetal Liver lineage negative, cKit+, Sca1+ cells were sorted from Hes-GFP mice for RNA extraction and hybridization on Affymetrix microarrays

ORGANISM(S): Mus musculus

SUBMITTER: Camille Lobry 

PROVIDER: E-GEOD-46725 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

Json Xml
altmetric image

Publications

Accumulating evidence suggests that Notch signaling is active at multiple points during hematopoiesis. Until recently, the majority of such studies focused on Notch signaling in lymphocyte differentiation and knowledge of individual Notch receptor roles has been limited due to a paucity of genetic tools available. In this manuscript we generate and describe animal models to identify and fate-map stem and progenitor cells expressing each Notch receptor, delineate Notch pathway activation, and per  ...[more]

Similar Datasets

Transcriptomics Expression data from adult LSK Hes1-GFP positive and adult LSK Hes1-GFP negative
2013-07-01 | E-GEOD-46722 | biostudies-arrayexpress
Transcriptomics Expression data from E13.5 Fetal Liver LSK Hes1-GFP positive and E13.5 Fetal Liver LSK Hes1-GFP negative
2013-07-01 | GSE46725 | GEO
Transcriptomics Expression data from adult Myeloerythroid Progenitors (MP) Hes1-GFP positive and adult Myeloerythroid Progenitors (MP) Hes1-GFP negative
2013-07-01 | E-GEOD-46723 | biostudies-arrayexpress
Transcriptomics Expression data from LSK WT and LSK NcstnKO
2011-05-12 | E-GEOD-27794 | biostudies-arrayexpress
Transcriptomics Expression data from LSK WT and LSK N1-C+
2011-05-12 | E-GEOD-27799 | biostudies-arrayexpress
Transcriptomics Expression data from adult LSK Hes1-GFP positive and adult LSK Hes1-GFP negative
2013-07-01 | GSE46722 | GEO
Transcriptomics Expression data from LSK WT, GMP WT and GMP NcstnKO
2011-05-12 | E-GEOD-27811 | biostudies-arrayexpress
Transcriptomics Expression data from adult Myeloerythroid Progenitors (MP) ICN2 positive and adult Myeloerythroid Progenitors (MP) ICN2 negative
2013-07-01 | E-GEOD-46724 | biostudies-arrayexpress
Transcriptomics Expression data from adult Myeloerythroid Progenitors (MP) Hes1-GFP positive and adult Myeloerythroid Progenitors (MP) Hes1-GFP negative
2013-07-01 | GSE46723 | GEO
Transcriptomics Notch Pathway Activation Targets AML Cell Homeostasis and Differentiation: THP1 cell line
2013-01-18 | E-GEOD-42259 | biostudies-arrayexpress