Unknown,Transcriptomics,Genomics,Proteomics

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Genome-wide profiling of microRNAs target transcripts in sweet orange


ABSTRACT: miRNAs-mediated gene silencing pathway plays vital roles in plant development, abiotic and biotic stress responses. Here, we carried out a high-throughput sequencing approach to identify miRNAs targets in leaves, flowers and fruit of sweet orange.C onsequently, 55257, 62365 and 19393 degraded mRNA fragments were identified in leaf, flower and fruit, respectively. miRNAs-mediated degraded fragments sequencing of the leaves, flowers and fruit in sweet orange

ORGANISM(S): Citrus sinensis

SUBMITTER: Liu Yuanlong 

PROVIDER: E-GEOD-46764 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Genome-wide comparison of microRNAs and their targeted transcripts among leaf, flower and fruit of sweet orange.

Liu Yuanlong Y   Wang Lun L   Chen Dijun D   Wu Xiaomeng X   Huang Ding D   Chen Lingling L   Li Li L   Deng Xiuxin X   Xu Qiang Q  

BMC genomics 20140820


<h4>Background</h4>In plants, microRNAs (miRNAs) regulate gene expression mainly at the post-transcriptional level. Previous studies have demonstrated that miRNA-mediated gene silencing pathways play vital roles in plant development. Here, we used a high-throughput sequencing approach to characterize the miRNAs and their targeted transcripts in the leaf, flower and fruit of sweet orange.<h4>Results</h4>A total of 183 known miRNAs and 38 novel miRNAs were identified. An in-house script was used t  ...[more]

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