Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

The Making of a Slicer: Activation of Human Argonaute-1


ABSTRACT: The Argonautes are the central protein component in small RNA silencing pathways. Of the four human Argonautes (hAgo1-4) only hAgo2 is an active slicer. We determined the structure of hAgo1 bound to endogenous copurified RNAs to 1.75 Å resolution and hAgo1 loaded with let-7 miRNA to 2.1 Å. Both structures are strikingly similar to the structures of hAgo2. A conserved DEDH catalytic tetrad within the PIWI domain of hAgo2 is required for its slicing activity. hAgo3, with an intact tetrad, could be activated by swapping in the N domain of hAgo2, without additional PIWI domain mutations. An additional mutation on a loop adjacent to the active site of hAgo1 results in slicer activity that is substantially enhanced by swapping in the N domain of hAgo2. Intriguingly, the elements that make Argonaute an active slicer involve a sophisticated interplay between the active site and more distant regions of the enzyme. RNAsprofiling of small RNAs associated with recombinant protein used for chrystallographic studies

ORGANISM(S): Spodoptera frugiperda

SUBMITTER: Astrid Haase 

PROVIDER: E-GEOD-46870 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

Similar Datasets

2013-05-20 | GSE46870 | GEO
2013-02-04 | E-GEOD-34032 | biostudies-arrayexpress
2013-02-04 | GSE34032 | GEO
2013-06-20 | E-GEOD-48076 | biostudies-arrayexpress
2012-09-28 | GSE40256 | GEO
2012-09-28 | E-GEOD-40258 | biostudies-arrayexpress
2012-09-28 | GSE40258 | GEO
2014-06-30 | GSE49436 | GEO
2012-09-28 | E-GEOD-40256 | biostudies-arrayexpress
2013-06-20 | GSE48076 | GEO