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Differentially expressed transcripts in SLE blood monocytes

ABSTRACT: To better characterize the molecules that could potentially confer antigen presenting capacity to SLE monocytes, we assessed their gene expression profile. Blood monocytes from five healthy controls and five pediatric SLE patients were isolated using CD14+ selection. Because drugs used to treat SLE could induce considerable transcriptional changes, we selected active, newly diagnosed patients who had never received oral or intravenous (IV) medications.

ORGANISM(S): Homo sapiens

SUBMITTER: Nicole Baldwin

PROVIDER: E-GEOD-46907 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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IFN priming is necessary but not sufficient to turn on a migratory dendritic cell program in lupus monocytes.

Rodriguez-Pla Alicia A Patel Pinakeen P Maecker Holden T HT Rossello-Urgell Jose J Baldwin Nicole N Bennett Lynda L Cantrell Victoria V Baisch Jeanine J Punaro Marilynn M Gotte Alisa A Nassi Lorien L Wright Tracey T Palucka Anna Karolina AK Banchereau Jacques J Pascual Virginia V

Journal of immunology (Baltimore, Md. : 1950) 20140514 12

Blood monocytes from children with systemic lupus erythematosus (SLE) behave similar to dendritic cells (DCs), and SLE serum induces healthy monocytes to differentiate into DCs in a type I IFN-dependent manner. In this study, we found that these monocytes display significant transcriptional changes, including a prominent IFN signature, compared with healthy controls. Few of those changes, however, explain DC function. Exposure to allogeneic T cells in vitro reprograms SLE monocytes to acquire DC ...[more]

PMID: 24829414

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2014-06-03 | E-GEOD-46911 | biostudies-arrayexpress

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2014-06-03 | E-GEOD-46923 | biostudies-arrayexpress

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2016-03-31 | E-GEOD-65391 | biostudies-arrayexpress

Gene profiling reveals specific molecular pathways in the pathogenesis of atherothrombosis in Antiphospholipid syndrome, Systemic Lupus Erythematosus and Lupus with Antiphospholipid Syndrome

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2014-03-12 | E-GEOD-50395 | biostudies-arrayexpress

Human colon expression in healthy controls, colon-only CD, ileo-colonic CD, and UC

Project description:Colon gene expression in human IBD. The three major clinical subsets of Inflammatory Bowel Disease (IBD) include colon-only Crohn's Disease (CD), ileo-colonic CD, and Ulcerative Colitis (UC). These experiments tested differential colon gene expression in these three types of IBD, relative to healthy control samples, and the local degree of mucosal inflammation as measured by the CD Histological Index of Severity (CDHIS). Colon biopsy samples were obtained from IBD patients at diagnosis and during therapy, and healthy controls. The global pattern of gene expression was determined using GeneSpring software, with a focus upon candidate genes identified in a recent genome wide association study in pediatric onset IBD. Data suggested that two of these candidate genes are up regulated in pediatric IBD, partially influenced by local mucosal inflammation. These experiments tested differential colon gene expression in healthy, CD, and UC samples for candidate genes identified in a recent pediatric onset IBD genome wide association study. Keywords: Single time point in CD and UC and healthy controls. Colon RNA was isolated from biopsies obtained from CD and UC at diagnosis and during therapy and healthy controls. Samples were obtained from the most proximal affected segment of colon. Microarray experiments were performed as described in the CCHMC microarray core, and data was analyzed as described above in the summary. The '107' internal control CEL files (for batches 1,2,3,4,5) used for normalization of the Sample VALUEs are also contained within this data set.

2009-03-10 | E-GEOD-10616 | biostudies-arrayexpress

Human colon expression in healthy controls and UC

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