Transcription profiling of murine macrophage-like RAW 264.7 cells treated with EdTx, protective antigen, or LPS for different length of time to examine the effect of EdTx on global transcriptional profiles.

ABSTRACT: Edema toxin (EdTx), which is a combination of edema factor and a binding moiety (protective antigen), is produced by Bacillus anthracis, the etiological agent of anthrax. EdTx is an adenylyl cyclase enzyme that converts adenosine triphosphate to adenosine-3',5'-monophosphate, resulting in interstitial edema seen in anthrax patients. We used GeneChip analysis to examine global transcriptional profiles of EdTx-treated RAW 264.7 murine macrophage-like cells at 3 and 6 hr. Experiment Overall Design: RAW 264.7 cells were treated with EdTx (2.5 µg/ml of protective antigen and 0.625 µg/ml of Edema factor), PA (2.5 µg/ml), or LPS (1 ng/mL) for 0, 3, and 6 hr. Each experiment was performed in triplicate, generating a total of 21 arrays (biological replciates).

ORGANISM(S): Mus musculus

SUBMITTER: Ashok Chopra 

PROVIDER: E-GEOD-4712 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress