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Hormonal Regulation of MicroRNA Expression in Rat Adrenal Gland

ABSTRACT: To determine miRNA profiles in rat adrenals from animals treated with vehicle, ACTH, 17M-NM-1-ethinyl estradiol (17M-NM-1-E2) or dexamethasone (DEX). miRNA expression profiling was performed using AffymetrixM-BM-. GeneChipM-BM-. miRNA 2.0 Arrays. Together with qRT-PCR, several adrenal miRNAs have been identified, whose expression is subject to regulation by one or more than one hormone. RNA was extracted from four group rat adrenal gland and miRNA profiles were established using microarray using AffymetrixM-BM-. GeneChipM-BM-. miRNA 2.0 Arrays and confirmed with qRT-PCR. The microarray data were analyzed using PartekM-BM-. Genome Suite software, version 6.3 CopyrightM-BM-) 2008 (Partek Inc., St. Louis, MO, USA) to comprehensively evaluate the differential expression level of the various samples.

ORGANISM(S): Rattus norvegicus

SUBMITTER: Wen-Jun Shen

PROVIDER: E-GEOD-47131 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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