Unknown,Transcriptomics,Genomics,Proteomics

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Tissue-specific pioneer factors associate with androgen receptor cistromes and transcription programs. [ChIP-Seq]


ABSTRACT: We report the in vivo androgen receptor (AR) binding sites in murine prostate, epididymis and kidney in response to physiological androgen testosterone using ChIP-sequencing and gene expression profiling by microarray. From AR cistrome analysis, we identified tissue-specific collaborating factors i.e. FoxA1 in prostate, Hnf4a in kidney and AP2a in epididymis and validated by ChIP-seq. The ChIP experiments have been performed using antibodies specific to AR, FoxA1, Hnf4a, AP-2a and IgG non-specific antibody as a negative control. Examination of AR binding sites in murine androgen-responsive tissues prostate, epididymis and kidney using ChIP-seq. Further analysis of AR cistromes led to identification of tissue-specific collaborating factors and these collaborating factors are validated by ChIP-seq from the same tissues. Two parallel IgG samples were sequenced, merged together and used as a control data set. Parallel ChIP-seq samples were sequenced and merged for each replicate wherever required to contain approximately the same amount of reads across all tissues and conditions. All ChIP-seq experiments are performed in biological duplicates except for the castrated conditions.

ORGANISM(S): Mus musculus

SUBMITTER: Olli Jänne 

PROVIDER: E-GEOD-47192 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Tissue-specific pioneer factors associate with androgen receptor cistromes and transcription programs.

Pihlajamaa Päivi P   Sahu Biswajyoti B   Lyly Lauri L   Aittomäki Viljami V   Hautaniemi Sampsa S   Jänne Olli A OA  

The EMBO journal 20140122 4


Androgen receptor (AR) binds male sex steroids and mediates physiological androgen actions in target tissues. ChIP-seq analyses of AR-binding events in murine prostate, kidney and epididymis show that in vivo AR cistromes and their respective androgen-dependent transcription programs are highly tissue specific mediating distinct biological pathways. This high order of tissue specificity is achieved by the use of exclusive collaborating factors in the three androgen-responsive tissues. We find tw  ...[more]

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