Androgen receptor uses relaxed response element stringency for selective chromatin binding and transcriptional regulation in vivo

ABSTRACT: We report the in vivo androgen receptor (AR) binding sites in caput epididymis of intact SPARKI and wild-type mice using ChIP-sequencing. SPARKI (specificity affecting androgen receptor knock-in) mouse line has the second zinc finger of AR replaced by that of glucocorticoid receptors. In vivo analysis of SPARKI and wild-type AR genome-wide binding sites identified cis-element with less stringent sequence requirements that specify selective AR-binding sites. The ChIP experiments have been performed using antibody specific to AR and IgG non-specific antibody as a negative control.

ORGANISM(S): Mus musculus

PROVIDER: GSE51106 | GEO | 2014/01/21

SECONDARY ACCESSION(S): PRJNA221193

REPOSITORIES: GEO