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Expression analysis of mock- or RAD21-transduced Kasumi1 cells

ABSTRACT: We recently identified recurrent mutations of cohesin complex in myeloid neoplasms through whole-exome sequencing analysis. RAD21 is one of the main components of the cohesin complex. In this study, to investigate the biological impact of wild-type RAD21 on Kasumi1 cells harboring RAD21 mutation, Kasumi1 cells were retrovirally transduced with either mock or wild-type RAD21, and expression array was performed. Expression analysis was performed for mock- or wild-type RAD21-transduced Kasumi-1 cells in triplicate. The experiment was performed twice independently.

ORGANISM(S): Homo sapiens

SUBMITTER: Ayana Kon

PROVIDER: E-GEOD-47641 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Expression analysis of mock- or RAD21-transduced Kasumi1 cells

Project description:We recently identified recurrent mutations of cohesin complex in myeloid neoplasms through whole-exome sequencing analysis. RAD21 is one of the main components of the cohesin complex. In this study, to investigate the biological impact of wild-type RAD21 on Kasumi1 cells harboring RAD21 mutation, Kasumi1 cells were retrovirally transduced with either mock or wild-type RAD21, and expression array was performed.

2013-07-16 | GSE47641 | GEO

RAD21-TEV NLS-TEV RNAseq - extended cohesin depletion

Project description:RNA-seq of vehicle and doxycycline treated Rad21-TEV primary neuron transduced with NLS-TEV, inducing extended cohesin depletion

2021-02-28 | GSE161797 | GEO

Cohesin regulates gene expression through spatial clustering of enhancer elements

Project description:H3K27Ac ChIP-seq in wild type and cohesin-deficient thymocytes Rad21 was deleted in CD4+ CD8+ double positive (DP) thymocytes by crossing a Rad21 floxed allele with a Cd4-driven Cre transgene. DP positive thymocytes were FACS-sorted from control and Rad21-/- littermates, which were then used to perform chromatin immunoprecipitation for histone H3 acetylated on lysine 27 (H3K27Ac).

2015-02-12 | E-GEOD-61428 | biostudies-arrayexpress

Rad21-mediated NF-κB signaling limits hematopoietic stem cell self-renewal in aging and inflammation

Project description:MTD project_description Inflammation and decreased stem cell function characterize organism aging, yet the relationship between these factors remains incompletely understood. This study shows that aged hematopoietic stem and progenitor cells exhibit increased ground-stage NF-κB activity, which enhances their responsiveness to undergo differentiation and loss of self-renewal in response to inflammation. The study identifies Rad21/cohesin as a critical mediator of NF-κB signals, by increasing chromatin accessibility of inter-/intra-genic and enhancer regions. Rad21/NF-κB are required for normal differentiation, but limit self-renewal of hematopoietic stem cells (HSCs) during aging and inflammation in an NF-κB dependent manner. HSCs from aged mice fail to downregulate Rad21/cohesin and inflammation/differentiation inducing signals in the resolution phase after acute inflammation. and The inhibition of cohesin/NF-κB is sufficient to revert the hypersensitivity of aged HSPCs to inflammation-induced differentiation. During aging, myeloid-biased HSCs with disrupted and naturally occurring reduced expression of Rad21/cohesin are increasingly selected over lymphoid-biased HSCs. Together, Rad21/cohesin mediated NF-κB signaling limits HSPC function during aging and selects for cohesin deficient HSCs with myeloid skewed differentiation.

2018-11-28 | PXD011537 | Pride

Chromatin immunoprecipitation of mouse B3 pre-B cells and VL3 thymocytes to identify cohesin and CTCF binding sites

Project description:Identification of cohesin (Rad21) and CTCF binding sites in ~2% of the mouse genome in B3 pre-B cells stably expressing FLAG-tagged Rad21 using anti-FLAG (Sigma F3165) and anti-CTCF (Upstate 07729) antibodies

2008-01-31 | E-TABM-401 | biostudies-arrayexpress

Rad21

Project description:Rattus norvegicus Rad21, RAD21 cohesin complex component [Source:RGD Symbol;Acc:1594529], is differentially expressed in 23 experiment(s);

| ENSRNOG00000004420 | ExpressionAtlas

RAD21

Project description:Monodelphis domestica RAD21, RAD21 cohesin complex component [Source:NCBI gene;Acc:100025333], is expressed in 2 baseline experiment(s);

| ENSMODG00000004133 | ExpressionAtlas

Rad21

Project description:Rattus norvegicus Rad21, RAD21 cohesin complex component [Source:RGD Symbol;Acc:1594529], is expressed in 6 baseline experiment(s);

| ENSRNOG00000004420 | ExpressionAtlas

RAD21

Project description:Sus scrofa RAD21, RAD21 cohesin complex component [Source:VGNC Symbol;Acc:VGNC:92054], is expressed in 5 baseline experiment(s);

| ENSSSCG00000006014 | ExpressionAtlas

rad21

Project description:Xenopus tropicalis rad21, RAD21 cohesin complex component [Source:Xenbase;Acc:XB-GENE-1008099], is expressed in 1 baseline experiment(s);

| ENSXETG00000020783 | ExpressionAtlas

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